May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Generation and characterization of a transgenic mouse over–expressing XIAP (X–linked inhibitor of apoptosis protein)
Author Affiliations & Notes
  • C. Tsilfidis
    Ophthalmology,
    University Ottawa Eye Inst, Ottawa, ON, Canada
  • A. Baker
    Ophthalmology,
    University Ottawa Eye Inst, Ottawa, ON, Canada
  • D. Petrin
    University Ottawa Eye Inst, Ottawa, ON, Canada
  • R. Dunkley
    Children's Hospital of Eastern Ontario, Ottawa, ON, Canada
  • R. Korneluk
    Children's Hospital of Eastern Ontario, Ottawa, ON, Canada
  • P. Liston
    Children's Hospital of Eastern Ontario, Ottawa, ON, Canada
  • Footnotes
    Commercial Relationships  C. Tsilfidis, None; A. Baker, None; D. Petrin, None; R. Dunkley, None; R. Korneluk, Aegera Therapeutics C; P. Liston, None.
  • Footnotes
    Support  Canadian Institutes of Health Research
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 771. doi:
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      C. Tsilfidis, A. Baker, D. Petrin, R. Dunkley, R. Korneluk, P. Liston; Generation and characterization of a transgenic mouse over–expressing XIAP (X–linked inhibitor of apoptosis protein) . Invest. Ophthalmol. Vis. Sci. 2004;45(13):771.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To generate a transgenic mouse which over–expresses an anti–apoptotic gene in the retina, for use in therapeutic crosses with retinal degeneration mice. Methods: A transgenic mouse was engineered which carries myc–tagged human XIAP driven by the ubiquitin promoter. The over–expression of XIAP in the retina was confirmed using genotyping, western analysis and immunohistochemistry. Retinal function was assessed using full–flash electroretinography (ERG). Results: Over–expression of XIAP is seen in western analysis of whole retina. There is approximately a 3–fold over–expression of the transgene in comparison to endogenous mouse IAP. Immunohistochemistry with the myc tag confirms that the XIAP transgene is found in the ganglion cell layer, the inner nuclear layer and the outer nuclear layer. Lamination of the retina appears to be normal. ERG analysis shows normal function in the transgenic retina in comparison to wild–type controls. Conclusions: The ubiquitin–XIAP mouse provides an excellent opportunity to test the effects of anti–apoptotic therapy on mouse models of retinal deneration.

Keywords: apoptosis/cell death • neuroprotection 
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