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F. Ishikawa, H. Ohguro, I. Ohguro, K. Mamiya, T. Metoki, H. Yamazaki, Y. Takano, T. Ito, M. Nakazawa; Study of photo–stress effects on normal and retinal degeneration rats . Invest. Ophthalmol. Vis. Sci. 2004;45(13):781.
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Purpose: The Royal College of Surgeons (RCS) rat is the most extensively characterized animal model for study of the molecular pathology of inherited retinal degeneration, such as retinitis pigmentosa (RP). The purpose of the present study is to study effects of light stress toward RCS rat retinal degeneration. Methods:: RCS rats and control rats (Sprague Dawley (SD), Brown Norway (BN)) were exposed with several deferent light stress (650, 1300, 2500 and 5000 lux) for 24 or 48hrs and thereafter retinal morphology and functions (electroretinogram (ERG) and rhodopsin (Rho) dephosphorylation) were analyzed. Results:In control rats, ERG response was significantly deteriorated, Rho dephosphorylation was marked prolonged, and apoptosis related factors and anti–apoptotic factors were up–regulated and down–regulated, respectively, in TaqMan ®PCR analysis were after a day from the light stress under strong light stress (2500 and 5000 lux) but no effects were observed in mild light stress (650 and 1300 lux). However, until following 7 to 14 days, ERG responses were recovered and its recovery was faster and more significant in BN rats than SD rats. In RCS rats, strong or mild light stress caused similar effects as above, but any recovery was observed in ERG response. However in contrast, TaqMan ®PCR analysis revealed that some anti–apoptotic factors and apoptotic factor were up–regulated and down regulated, respectively, in RCS rat exposed with mild light stress. Conclusions: Based upon these data, it is suggested that mild light stress may be beneficial for the preservation of photoreceptor cells in RCS rats.
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