May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The effect of erythropoietin on hyperoxia–induced retinal degeneration and hypoxia–induced retinal neovascularization.
Author Affiliations & Notes
  • J. Sung
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • R. Lima e Silva
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • Y. Saishin
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • M. Zhang
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • B.S. Rogers
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • T.G. Deering
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • S. Aslam
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • Y. Saishin
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • P.A. Campochiaro
    Ophthalmology, Johns Hopkins University, Baltimore, MD
  • Footnotes
    Commercial Relationships  J. Sung, None; R. Lima e Silva, None; Y. Saishin, None; M. Zhang, None; B.S. Rogers, None; T.G. Deering, None; S. Aslam, None; Y. Saishin, None; P.A. Campochiaro, Warren Pharmaceuticals F.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 839. doi:
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      J. Sung, R. Lima e Silva, Y. Saishin, M. Zhang, B.S. Rogers, T.G. Deering, S. Aslam, Y. Saishin, P.A. Campochiaro; The effect of erythropoietin on hyperoxia–induced retinal degeneration and hypoxia–induced retinal neovascularization. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):839.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To determine the effect of erythropoietin (EPO1) and its derivatives (EPO2 & EPO3) in mouse models of hyperoxia–induced retinal degeneration and hypoxia–induced retinal neovascularization. Methods: Hyperoxia–induced retinal degeneration model: Adult wild–type mice were administered erythropoietin, vehicle (phosphate buffered saline (PBS)), or no injections and placed in 70% oxygen for 3 weeks. Measurements of outer nuclear layer (ONL) and electroretinograms (ERG) were performed. Hypoxia–induced retinal neovascularization model: Wild–type mouse pups were placed in 75% oxygen from P7 to P12. Pups were injected with EPO1, 2 & 3 or PBS. Total area of retinal neovascularization was measured. Results: Hyperoxia–induced retinal degeneration model: Mice treated with EPO1 showed preservation of the a–wave amplitude (p=0.02) whereas those treated with EPO2 showed preservation of the b–wave amplitude (p=0.05). Mice injected with eythropoietin and PBS showed survival of the ONL. Hypoxia–induced retinal neovascularization model: Mouse pups treated with EPO2 exhibited decreased areas of retinal neovascularization (p=0.05). Conclusions: Erythropoietin and its derivatives appear to promote survival and maintain retinal function in mouse eyes subjected to oxidative damage. EPO2, an erythropoietin derivative also appears to decrease the stimulus for retinal neovascularization in the hypoxia–induced mouse model.

Keywords: neuroprotection • neovascularization • oxidation/oxidative or free radical damage 
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