May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The neuroprotective effect of hyperbaric oxygen treatment on laser–induced retinal damage inrats.
Author Affiliations & Notes
  • V. Vishnevskia–Dai
    Ophthalmology Department,
    Assaf Harofeh Medical Center Tel Aviv University, Zerifin, Israel
  • M. Belokopytov
    Goldschleger Eye Research Institute, Sheba Medical Center Tel Aviv University, Ramat Gan, Israel
  • G. Dubinsky
    Goldschleger Eye Research Institute, Sheba Medical Center Tel Aviv University, Ramat Gan, Israel
  • G. Nahum
    The Hyperbaric Institute,
    Assaf Harofeh Medical Center Tel Aviv University, Zerifin, Israel
  • M. Rosner
    Goldschleger Eye Research Institute, Sheba Medical Center Tel Aviv University, Ramat Gan, Israel
  • I. Avni
    Ophthalmology Department,
    Assaf Harofeh Medical Center Tel Aviv University, Zerifin, Israel
  • M. Belkin
    Goldschleger Eye Research Institute, Sheba Medical Center Tel Aviv University, Ramat Gan, Israel
  • Footnotes
    Commercial Relationships  V. Vishnevskia–Dai, None; M. Belokopytov, None; G. Dubinsky, None; G. Nahum, None; M. Rosner, None; I. Avni, None; M. Belkin, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 847. doi:
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      V. Vishnevskia–Dai, M. Belokopytov, G. Dubinsky, G. Nahum, M. Rosner, I. Avni, M. Belkin; The neuroprotective effect of hyperbaric oxygen treatment on laser–induced retinal damage inrats. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):847.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Background: Retinal damage induced by trauma ischemia or laser photocoagulation increases considerably by the secondary degeneration process whereby tissues adjacent to the primary lesion are destroyed. The histological and functional consequences of the injury can be ameliorated by neuroprotection. Hyperbaric Oxygen (HBO) treatment consists of breathing oxygen at higher than atmospheric pressure. Improved neural function was observed in patients after acute neural trauma or ischemia that were treated with HBO several hours after the initial insult, suggesting that HBO may have a neuroprotective effect. Objective: This study was designed to examine the effect of hyperbaric oxygen on laser induced retinal damage in a rat model. Materials and Methods: 25 Pigmented DA rats were irradiated with argon laser (514 nm 200µ spot at 0.1 MW for 0.05 sec) at different retinal points located 2 disc diameter from the optic discs. 1. Treatment Group (T): 10 rats were exposed immediately after damage to15 consecutive HBO treatments (100% O2 for 90 min at 2 (ATA )Absolute Atmospheric pressure) , X 3 /first 24 hr followed by 12 consecutive daily treatments. 2. Treatment Group (H): 5 rats were similarly exposed to 10 consecutive HBO treatments (100% O2 for 60 min at 2 ATA) 3. Control Group (O): 10 rats that had undergone laser damage, with no additional treatment. Damage assessment: 20 days after the injury a masked histopathological measurement of retinal lesions size and retinal cells count were performed. Cell density was calculated accordingly. Statistical analysis: two–tailed unpaired T–test Results: The lesion size was significantly smaller in the treatment groups as compared to the control group T=336µ; H=355µ; O=390µ, T–O P<0.01,H–O P<0.05. Cell density in the entire lesion and its center was significantly higher compared with the control group. *Entire lesion: T=83%,H=71%,O=65% T–O P< 0.01H–O P< 0.01. Center of lesion: T=68%,H=51%,O=31% T–O P<0.01,H–OP<0.01. * % of normal cell count in an un lasered tissue, adjacent to each lesion. Conclusions: According to this study, hyperbaric oxygen seems to have a neuroprotective effect on laser–induced retinal damage in a rat model. In the range of HBO exposures studied, higher exposure provides more neuroprotection. These results encourage further examination of the potential therapeutic use of hyperbaric oxygen in diseases and injuries of the retina.

Keywords: neuroprotection • retina • pathology: experimental 
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