May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Feline Immunodeficiency Virus Vector Mediated Gene Transfer to the Retina In Vivo in Neonatal Rats
Author Affiliations & Notes
  • M.S. Good
    Ophthalmology, Mayo Clinic, Rochester, MN
  • K.G. Howell
    Ophthalmology, Mayo Clinic, Rochester, MN
  • C.A. McCannel
    Ophthalmology, Mayo Clinic, Rochester, MN
  • Footnotes
    Commercial Relationships  M.S. Good, None; K.G. Howell, None; C.A. McCannel, None.
  • Footnotes
    Support  American Glaucoma Society, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 878. doi:
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    • Get Citation

      M.S. Good, K.G. Howell, C.A. McCannel; Feline Immunodeficiency Virus Vector Mediated Gene Transfer to the Retina In Vivo in Neonatal Rats . Invest. Ophthalmol. Vis. Sci. 2004;45(13):878.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The purpose of this study was to evaluate feline immunodeficiency virus (FIV)–based vector mediated gene transfer to the retina in vivo in neonatal rats. Methods: FIV vector mediated gene transfer to the retina in vivo was evaluated in neonatal Sprague Dawley rats using intravitreal and subretinal injections of FIV vector (1 x 109 TU/ml) containing the lacZ reporter gene. One group of rats received an intravitreal injection of 5ul of FIV vector through the pars plana of one eye. The second group of rats received a subretinal injection of 5ul of FIV vector. The fellow eye was not injected and served as the control. X–gal staining was used to determine lacZ expression at two weeks. Results: In neonatal rats, eyes injected intravitreally with FIV vector containing the lacZ reporter gene show blue X–gal staining localized to the injection site at two weeks. Retinal cross sections show transduction of the RGCs. No lacZ expression was present in the fellow (control) eyes. Eyes injected subretinally with FIV vector containing the lacZ reporter gene show diffuse blue X–gal staining at two weeks. Retinal cross sections show high efficiency transduction of the retinal pigment epithelium (RPE). No lacZ expression was present in the fellow (control) eyes. Conclusions: In neonatal rats, RGCs and RPE were successfully transduced in vivo using FIV vector containing the lacZ reporter gene. Intravitreal injections of FIV vector result in localized transduction of the RGCs and subretinal injections of FIV vector result in diffuse transduction of the RPE.

Keywords: neuroprotection • ganglion cells • gene transfer/gene therapy 
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