May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
DOWN REGULATION OF APOPTOSIS RELATED GENE EXPRESSION AND ACTIVATION OF NF–kB BY NIPRADILOL DURING SERUM DEPRIVATION–INDUCED APOPTOSIS IN PC12 CELLS
Author Affiliations & Notes
  • A. Ando
    Ophthalmology, Kansai Med University, Moriguchi, Japan
  • Y. Yamazaki
    Ophthalmology, Kansai Med University, Moriguchi, Japan
  • S. Kaneko
    Ophthalmology, Kansai Med University, Moriguchi, Japan
  • M. Matsumura
    Ophthalmology, Kansai Med University, Moriguchi, Japan
  • Footnotes
    Commercial Relationships  A. Ando, KOWA COMPANY, LTD F; Y. Yamazaki, None; S. Kaneko, None; M. Matsumura, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 886. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Ando, Y. Yamazaki, S. Kaneko, M. Matsumura; DOWN REGULATION OF APOPTOSIS RELATED GENE EXPRESSION AND ACTIVATION OF NF–kB BY NIPRADILOL DURING SERUM DEPRIVATION–INDUCED APOPTOSIS IN PC12 CELLS . Invest. Ophthalmol. Vis. Sci. 2004;45(13):886.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Nipradilol is an α1 ß blocker that is used as an antiglaucomatous agent and it has a cytoprotective effect in neuronal cells. Recently, it was reported that decrease of caspase–3 activity resulting in inhibition of apoptosis is implied in the mechanism of cytoprotection. However, whether nipradilol regulates gene expression is not known. We tried to clarify the effects of nipradilol on the expressions of apoptosis associated genes and the activation of NF–kappaB during serum deprivation–induced apoptosis in PC12 cells. Methods: PC12 cells were cultured in serum free RPMI1640 medium with 0.01, 0.1, 1, 10 µM or without nipradilol. Cells were cultured in medium with serum for controls. Gene expression of Bax, Bcl–2, Fas, FasL, Caspase–1, 2, 3 and 9, p53 and smac/DIABLO were examined by quantitative real time polymerase chain reaction. Translocation into nucleus of NF–kappaB was examined by electrophoresis mobility shift assay using NF–kappaB consensus sequenced DNA probe. Results: Gene expressions of Bax and capase–3 and 9 were upregulated during serum deprivention–induced apoptosis in PC12 cells compared to serum controls. 0.1 and 1 µM of nipradilol suppressed expression of Bax, capase–3 and 9 and smac/DIABLO gene. Expressions of the other genes examined in this experiment were not affected by nipradilol. Translocation into nucleus of NF–kappaB was increased by 0.1 and 1 µM of nipradilol. Conclusions: These data suggest that nipradilol could regulate the apoptosis associated gene expression and NF–kappaB activation during prevention of apoptosis.

Keywords: apoptosis/cell death • neuroprotection • nitric oxide 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×