May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The Study For Orientation Of Limbal Epithelial Cell Movement In Rabbits
Author Affiliations & Notes
  • S.–H. Choi
    Ophthalmology, Chungnam National Univ Hosp, Taejon, Republic of Korea
  • Y.–W. Lee
    Ophthalmology, Chungnam National Univ Hosp, Taejon, Republic of Korea
  • C.–S. Kim
    Ophthalmology, Chungnam National Univ Hosp, Taejon, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Choi, None; Y. Lee, None; C. Kim, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1437. doi:
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    • Get Citation

      S.–H. Choi, Y.–W. Lee, C.–S. Kim; The Study For Orientation Of Limbal Epithelial Cell Movement In Rabbits . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1437.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the orientation of limbal epithelial cell movement in rabbits. Methods: We performed the 360 degree, 2 mm width autologous limbal transplantation in situ(directed corneal end toward cornea and conjunctival end toward conjunctiva) and in versus manner. We observed the cornea using slit lamp, light microscope, immunohistochemical and immunofluorescence stain, and confocal microscope. Results: Under slit lamp, cornea appeared no new vessels and clear in both manner. No goblet cells and normal epithelial arrangement were showed under LM. Positive stains for cornea specific AK2 and AE5 and negative stains for goblet cell–specific AM3 were noted in both manner. Under confocal microscope, dye–uptaken limbal epithelial cells moved toward corneal center in both specimens. Conclusions: We confirmed that limbal epithelial cells in rabbits have the uniform nature of moving toward corneal center at any positions.

Keywords: cornea: epithelium 
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