May 2004
Volume 45, Issue 13
ARVO Annual Meeting Abstract  |   May 2004
Protein Protection of Corneal Epithelium from Rose Bengal and Lissamine Green
Author Affiliations & Notes
  • L.–J. Lai
    Department of Ophthalmology, Chang Gang Memorial Hospital, Taipei, Taiwan Republic of China
  • G.N. Foulks
    Kentucky Lions Eye Center, University of Louisville, Louisville, KY
  • N. SundarRaj
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA
  • Footnotes
    Commercial Relationships  L. Lai, None; G.N. Foulks, None; N. SundarRaj, None.
  • Footnotes
    Support  CMRPG32011 (Taiwan)
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1457. doi:
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      L.–J. Lai, G.N. Foulks, N. SundarRaj; Protein Protection of Corneal Epithelium from Rose Bengal and Lissamine Green . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1457.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To evaluate the growth effect and protein protective effect of albumin and mucin for human corneal epithelium upon exposure to rose bengal dye and lissamine green dye in vitro. Methods: Human corneal epithelium obtained from corneoscleral rims of donor tissue was cultured by a standard technique. Mucin (200 ug/ml) or 1% albumin was added in the supplemental hormonal epithelial medium (SHEM) for this experiment. Vital dyes of 1% lissamine green or 1% rose bengal were exposed to cells to evaluate the protective effect of selected proteins. Immunohistochemistry stain of Ki67 was preformed to evaluate the growth effect from albumin and mucin. Transmission electromicroscopy was done to evaluate the microstructure of corneal epithelium. Results: Human corneal epithelium grows faster in the culture medium containing 1% albumin compared with the control group (p=0.032) and there is no significant difference between the media containing mucin and the control group (p=0.21). Cell viability was 93.6% for 1% lissamine green in SHEM medium, 87.5% in SHEM media containing 1% albumin, and 90.5% in SHEM media contain mucin. Cell viability was 9.6% for 1% rose bengal in SHEM medium, 6.2% in SHEM media containing 1% albumin, and 30.7% in SHEM media contain mucin. Immunohistochemistry stain of Ki67 showed the prominent growth of corneal epithelium in the media with albumin. Conclusions: Albumin promoted corneal epithelial cells to grow faster in vitro, but did not have protective effect from drug toxicity. Mucin formed a more effective barrier and prevented corneal epithelial damage from toxicity of vital dyes such as rose bengal and lissamine green.  

Keywords: cornea: epithelium • protein structure/function 

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