May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The Regulation of Transcription of PAI–2 by pRb2/p130 in Human Corneal and Conjunctival Epithelial Cells. M. Massaro–Giordano1A, M. Macaluso2, M. Montanari,2 C.M. Marshall1B, A. Giordano2. Dept of OphthalmologyA, Dept of DermatologyB, University of Pennsylvania, Philadelphia PA1. Temple University, Philadelphia, PA2
Author Affiliations & Notes
  • M. Massaro–Giordano
    Ophthalmology, Scheie Eye Institute, Philadelphia, PA
  • Footnotes
    Commercial Relationships  M. Massaro–Giordano, None.
  • Footnotes
    Support  Sbarro Health Research Organization
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1464. doi:
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      M. Massaro–Giordano; The Regulation of Transcription of PAI–2 by pRb2/p130 in Human Corneal and Conjunctival Epithelial Cells. M. Massaro–Giordano1A, M. Macaluso2, M. Montanari,2 C.M. Marshall1B, A. Giordano2. Dept of OphthalmologyA, Dept of DermatologyB, University of Pennsylvania, Philadelphia PA1. Temple University, Philadelphia, PA2 . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1464.

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Abstract

Abstract: : Purpose: Plasminogen activator inhibitor type 2 (PAI–2) is a serine proteinase inhibitor found in human stratified squamous epithelia (e.g., skin, oral mucosa, cornea). Several studies have shown that high levels of PAI–2 are associated with good prognosis in patients with breast cancer, small cell lung cancer and ovarian cancer. Recently, it has been reported that PAI–2 interacts with the Rb (retinoblastoma) protein by a novel Rb–binding motif and colocalizes with it in the nucleus. We would like to investigate if there is a functional interaction among PAI–2, pRb2/p130 (retinoblastoma related protein) and chromatin modifying enzymes in the regulation of PAI–2 gene transcription. Methods/ Results: We have explored the molecular mechanism regulating PAI–2 expression levels in normal cornea and conjunctiva. We found, by using chromatin crosslinked immunoprecipitation (XCHIP) assay, that both pRb2/p130 and PAI–2 proteins bind to a region of PAI–2 promoter . Conclusion: Our hypothesis is that the presence of pRb2/p130 and PAI–2 on PAI–2 promoter could regulate the transcription of this gene through the recruitment of different chromatin remodelling enzymes and the modulation of chromatin packaging. The results of this study can be important to understand the molecular mechanisms that dictate the different expression profiles of PAI–2 in the human corneal and conjunctival epithelial cells.

Keywords: cornea: epithelium • conjunctiva • cornea: basic science 
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