May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Immunocytologic detection of MUC5AC in ocular surface diseases
Author Affiliations & Notes
  • L. Racine
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX
  • Y. Chu
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX
  • W.J. Farley
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX
  • M. Jumblatt
    Ophthalmology, University of Louisville, Louisville, KY
  • S.C. Pflugfelder
    Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX
  • Footnotes
    Commercial Relationships  L. Racine, None; Y. Chu, None; W.J. Farley, None; M. Jumblatt, None; S.C. Pflugfelder, None.
  • Footnotes
    Support  NIH Grant EY11915; EY10736
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1492. doi:
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      L. Racine, Y. Chu, W.J. Farley, M. Jumblatt, S.C. Pflugfelder; Immunocytologic detection of MUC5AC in ocular surface diseases . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1492.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the expression of goblet cell mucin (MUC5AC) using immunocytology in normal controls and in patients with a variety of ocular surface diseases, including dry eye. Methods:Normal control patients and patients with Sjogren (SS KCS) and non–Sjogren (non SS KCS) keratoconjunctivitis sicca, Stevens–Johnson syndrome (SJS), superior limbic keratoconjunctivitis (SLK) and clinical signs of limbal stem cell deficiency diagnosis were recruited. Impression cytology of the conjunctiva and/or cornea was performed using Millipore Biopore membranes. MUC5AC in these specimens was immunodetected by indirect immunofluoresent staining. The number of MUC5AC positive goblet cells per high power field was counted. Unpaired t test was used to compare the mean number of MUC5AC positive goblet cells in specimens from controls and patients with ocular surface disease. Results:Four normal controls and ten patients with the following ocular surface disease were recruited: SS KCS (n=2), non SS KCS (n = 4), SJS (n = 2), SLK ( n =1), and aniridia (n =1). The mean number of MUC5AC positive conjunctival goblet cells was significantly reduced in SS/KCS (meanGCSS = 1.9 +/– 0.53) and SJS (meanSJS = 1.0 +/– 0.26; p = 0.0028) compared to controls (meanGCnormal = 6.45 +/– 1.16; p = 0.0119) and non SS/KCS (meannonSS = 12.8 +/– 1.68; p = 0.0001). Patients with non SS/KCS had more goblets cells than normal controls (p = 0.035). Corneal goblet cells were observed only in the aniridia specimen. Conclusions:Immunocytology can provide rapid and specific detection of goblet cell mucin MUC5AC in ocular surface diseases, including KCS and limbal stem cell deficiency. There appears to be distinct differences in the expression of MUC5AC between SS KCS and non–SS KCS.

Keywords: conjunctiva • cytology • cornea: surface mucins 
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