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J. Park, Y. Lee, J. Kim, Y. Shin, J. Kim; The Effect of Pinitol on Cataractogenesis in Streptozotocin Induced Diabetic Rats . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1705.
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Purpose: Pinitol, a natural extract from soybean, is metabolized to chiro–inositol in the body. We evaluated the effect of pinitol in inhibition of diabetic cataract from oxidative stress in streptozotocin(STZ) induced diabetic rats. Methods: We used 2 week–old, Sprague–Dawley rats (n=80). Diabetes was induced with STZ (60mg/kg, i.v.) and monitored by blood glucose level and weight of rats. These STZ–injected rats were applied pinitol or chiro–inositol, each 20mg/ml giving access to regular chow. Its efficacy was studied by monitoring a series of lens opacity and corneal lesion by photodocumentation. Blood samples, aqueous humor and lens samples were collected at the 2nd week, 4th week, 8th week and 12th week respectively. We measured glutathione, malondialdehyde(MDA) used ELISA technique to evaluate its antioxidant effect. The levels of polyol including sorbitol and chiro–inositol in aqueous humor and lens were measured by HPLC. Results: STZ–diabetic rats showed typical Y sutural lens opacity from the 3rd day and progressed diffusely more cortical opacity from the 1st week. But in pinitol treated diabetic rats, these cataractous changes were remarkably decreased. And also corneal edema and opacity reduced remarkably in this treated group. Blood glucose level reduced to 30∼40% in pinitol treated group. Glutathione level was markedly increased compared to that of nontreated diabetic group (15.59±1.44mM/mg, 5.57±0.49mM/mg, respectively). Which resulted in decrease of peroxidized MDA product in treated group. Also, the level of sorbitol decreased in treated group (2.7±0.11uM/g, 3.5±0.15uM/g, respectively). Similarly, the anti–cataractogenic and anti–oxidative effects of pinitol were also revealed in that of chiro–inositol treated group. Conclusions: These results suggest that pinitol could be effective in preventing cataract and cornea edema caused from oxidative stress in hyperglycemic environment.
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