May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Matrix Metalloproteinases and Metalloproteinase Inhibitors in normal Bruch's Membrane and Basal Laminar Deposits
Author Affiliations & Notes
  • P. Hermans
    Dept Ophthalmology, Ophtha–Lab at St. Franziskus–Hospital, Muenster, Germany
  • A. Lommatzsch
    at St Franziskus Hospital,
    Dept of Ophthalmology, Muenster, Germany
  • K.–D. Mueller
    University Duisburg–Essen, Dept of Microbiology, Essen, Germany
  • N. Bornfeld
    University Duisburg–Essen, Dept of Ophthalmology, Essen, Germany
  • D. Pauleikhoff
    at St. Franziskus Hospital,
    Dept of Ophthalmology, Muenster, Germany
  • Footnotes
    Commercial Relationships  P. Hermans, None; A. Lommatzsch, None; K. Mueller, None; N. Bornfeld, None; D. Pauleikhoff, None.
  • Footnotes
    Support  DFG Pa 357/5–2
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1788. doi:
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      P. Hermans, A. Lommatzsch, K.–D. Mueller, N. Bornfeld, D. Pauleikhoff; Matrix Metalloproteinases and Metalloproteinase Inhibitors in normal Bruch's Membrane and Basal Laminar Deposits . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1788.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The extracellular matrix (ECM) in Bruch's membrane (BM) changes with age due to increased crosslinking and incorporation of waste products. It is believed that an accumulation of ECM in the presence of waste material is responsible for drusen and basal laminar deposit (BLD) formation accompanied with a chronic low inflammatory response. The ECM turnover is modulated by degrading enzymes ( metalloproteinases MMP's) and their inhibitors ( tissue inhibitor of metalloproteinases TIMP's ), therefore the aim of this study was to determine the distribution of MMP's and TIMP's in normal macula Bruch's membrane of different age and in BLD. Methods: BM of 30 macula specimen of different age (<40y, 41–60y, <61y, each group 10 specimen) and 20 BLD containing CNV membranes were examined by the immunhistochemical ABC–method. Antibodies against MMP–2, MMP–7, MMP–9 and TIMP–1,TIMP–2, TIMP–3 were used. Immunoreactivity was resolved with horseradish peroxidase–aminoethylcarbazole, which produces a magenta reaction product. Negative control experiments were performed by omitting the first antibody. Results: In normal macula TIMP–2 could be detected at the surface of the RPE cells and the choriocapillary stroma (CC), while TIMP–3 was only present in BM. This staining intensity increased with age. In BLD specimen TIMP–2 was absent in the RPE and TIMP–3 was visible in the basal part of BLD. In contrast MMP's were not detectable in BM of normal macula, while MMP–7 was found in BLD specimen at the RPE surface and especially in BLD. Conclusions: In young and old BM predominantly TIMP–3 was found, while MMP's were only at the RPE detectable. In contrast BLD's contained MMP–7 as well as TIMP–3. In normal BM it appears to exist an equilibrium of ECM modulating proteins. The different pattern in the expression of ECM modulating proteins and especially the presence of MMP–7 in BLD can be interpreted as a switch in the balance between TIMP's and MMP's associated with the deposition of ECM proteins in BLD.

Keywords: extracellular matrix • Bruch's membrane • drusen 
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