May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Similarities in Oxidative Damage from AMD and Retinal Light Damage
Author Affiliations & Notes
  • K. Renganathan
    Ophthalmology, Cole Eye Inst i–31/CCF, Cleveland, OH
    Department of Chemistry, Case Western Reserve University, Cleveland, OH
  • R. Collier
    Alcon Inc, Fort Worth, TX
  • X. Gu
    Ophthalmology, Cole Eye Inst i–31/CCF, Cleveland, OH
  • R.G. Salomon
    Department of Chemistry, Case Western Reserve University, Cleveland, OH
  • M. Kapin
    Alcon Inc., Fort Worth, TX
  • J.G. Hollyfield
    Ophthalmology, Cole Eye Inst i–31/CCF, Cleveland, OH
  • J.W. Crabb
    Ophthalmology, Cole Eye Inst i–31/CCF, Cleveland, OH
    Cell biology, Lerner Research Institute, Cleveland, OH
  • Footnotes
    Commercial Relationships  K. Renganathan, None; R. Collier, Alcon Inc. F; X. Gu, None; R.G. Salomon, None; M. Kapin, Alcon Inc. F; J.G. Hollyfield, None; J.W. Crabb, None.
  • Footnotes
    Support  EY06603, EY14239, EY14240, GM21249, HL53315, FFB and CCF
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1795. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Renganathan, R. Collier, X. Gu, R.G. Salomon, M. Kapin, J.G. Hollyfield, J.W. Crabb; Similarities in Oxidative Damage from AMD and Retinal Light Damage . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1795.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To screen for similarities in oxidative stress mechanisms caused by retinal light damage and age–related macular degeneration (AMD) using rats and in vivo blue light exposure. Methods: Albino rats were dark adapted for 24 hrs. Control rats (N=10) remained in the dark while experimental rats (N=10) were exposed to blue light (220 fc) for 6 hours. Rats were sacrificed immediately following light treatment. Retinas were isolated, immediately washed with antioxidants and frozen at –80ºC until analysis. Lipids were extracted with chloroform/methanol and proteins were solubilized with SDS. Western analysis was used to screen for oxidative protein modifications. Results: Anti–carboxyethylpyrrole and anti–nitrotyrosine immunreactivities were significantly greater after 6h light exposure compared with control animals maintained in the dark. Carboxyethylpyrrole (CEP) adducts are derived from oxidation of docosahexaenoyl lipids (DHA–PC) and nitrotyrosine is generated from reactive nitrogen oxide species. Conclusions: Current results are consistent with our observations that green light stimulates in vivo protein nitration (2002 Mol. & Cell. Proteomics 1, 293) and CEP modifications in rat retina (2003 ARVO abstract 5129). Notably, AMD drusen/Bruch's membrane and light damaged rat retina both contain increased amounts of CEP adducts (2002 Proc Natl Acad Sci USA 99, 14682) and crystallin (2003 Exp Eye Res 76, 131). Rodent light damage models may be useful for validating therapies for AMD. CR: Y. Supported in part by NIH grants EY06603, EY14239, EY14240, GM21249, HL53315, The Foundation Fighting Blindness, The Cleveland Clinic Foundation and Alcon Inc.

Keywords: oxidation/oxidative or free radical damage • protein modifications–post translational • age–related macular degeneration 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×