May 2004
Volume 45, Issue 13
ARVO Annual Meeting Abstract  |   May 2004
Author Affiliations & Notes
  • B. Fardin
    Ophthalmology, University California–Irvine, Irvine, CA
  • R. Narayanan
    Ophthalmology, University California–Irvine, Irvine, CA
  • B.D. Kuppermann
    Ophthalmology, University California–Irvine, Irvine, CA
  • M.C. Kenney
    Ophthalmology, University California–Irvine, Irvine, CA
  • Footnotes
    Commercial Relationships  B. Fardin, None; R. Narayanan, None; B.D. Kuppermann, None; M.C. Kenney, None.
  • Footnotes
    Support  The Discovery Fund for Eye Research and the Skirball Molecular Ophthalmology Program
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1797. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B. Fardin, R. Narayanan, B.D. Kuppermann, M.C. Kenney; ACTIVATION OF CASPASE–12 BY OXIDIZED CHOLESTEROL IN ARPE–19 CELLS . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1797.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To investigate the involvement of caspase–12 in apoptosis induced by 7–ketocholesterol in human retinal pigment epithelial (ARPE–19) cells. Methods: ARPE–19 cells were cultured overnight in 60 mm culture dishes with Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum and then treated with 20 µg/ml of 7–ketocholesterol for 2 or 24 hours. Cell protein was extracted by lysis of cells with 1X CHAPS buffer and analyzed for activation of caspase–12 by Western blot analysis. Rabbit polyclonal antibody against activated caspase–12 (1:1000) was used for the Western blot. Results: An approximately 42 kDa band of cleaved caspase–12 was seen in ARPE–19 cells treated with 7–ketocholesterol at both 2 hours and 24 hours. There was a slight increase in the amount of activated caspase–12 at 24 hours compared to 2 hours. Cleaved caspase–12 was not seen in the untreated ARPE–19 cells. Conclusions: Previous studies showed that 7–ketocholesterol induced apoptosis in ARPE–19 cells. Caspase–12 is a representative of endoplasmic reticulum stress–induced cell–signaling apoptotic pathway. Our study demonstrated that the endoplasmic reticulum (ER) pathway of apoptosis may be occurring in the ARPE–19 cells as a result of 7–ketocholesterol treatment.

Keywords: retinal pigment epithelium • apoptosis/cell death 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.