May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Retinal up–regulation of purinergic P2X2 and P2Y2 receptors during hypoxia–induced retinopathy.
Author Affiliations & Notes
  • J.E. Gallo
    Ophthalmology,
    Facultad de Ciencias Biomedicas and Hospital Universitario Austral, Pilar – Buenos Aires, Argentina
  • A. Kvanta
    Ophthalmology, St Erik's Eye Hospital, Stockholm, Sweden
  • M.J. Villar
    Neuroscience,
    Facultad de Ciencias Biomedicas and Hospital Universitario Austral, Pilar – Buenos Aires, Argentina
  • I. van der Ploeg
    Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
  • S. Sarman
    Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
  • J.O. Croxatto
    Ocular Pathology, Fundacion Oftalmologica Argentina "Jorge Malbran", Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships  J.E. Gallo, None; A. Kvanta, None; M.J. Villar, None; I. van der Ploeg, None; S. Sarman, None; J.O. Croxatto, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1880. doi:
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      J.E. Gallo, A. Kvanta, M.J. Villar, I. van der Ploeg, S. Sarman, J.O. Croxatto; Retinal up–regulation of purinergic P2X2 and P2Y2 receptors during hypoxia–induced retinopathy. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1880.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Purinergic receptors have been found upregulated in retinal diseases. We aimed to analyse the expression of purinergic receptors in a mouse model of oxygen induced retinopathy Methods: Five newborn C57BL/6J mice were exposed to 75% oxygen for 5 days whereafter they were returned to normoxia for 5 days. Animals were euthanised and the eye balls extracted. A control group was made up of five mice. The retinas were analyzed using immunohistochemistry with P2X1, P2X2, P2X3, and P2Y2antibodies Results: P2X2 expression was found in the neovascular tufts and in the inner plexiform layer of the treated group. In addition, there was an increased density of P2Y2–stained cells in the nerve fiber layer. A positive immunoreactivity of P2X2 receptors was seen in the outer plexiform layer of the control and treated groups. Conclusions: Increased ATP responsiveness and glial cell proliferation after injury may be mediated by purinergic receptors. Purinergic P2X2 and P2Y2 receptors may play a role in hypoxia–induced retinal neovascularization

Keywords: retinopathy of prematurity • receptors • retina 
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