May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Identification of gene expression profiling associated with angiogenesis in human retinal endothelial cells compared with different endothelial cells.
Author Affiliations & Notes
  • H. Oharazawa
    Department of Ophthamology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • T. Igarashi
    Gene therapy section, National Eye Institute, National Institute of Health, Bethesda, MD
  • T. Kurai
    Department of Biochemistry and Molecular Biology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • H. Matsui
    Department of Ophthamology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • T. Shiwa
    Department of Ophthamology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • H. Takahashi
    Department of Ophthamology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • K. Ohara
    Department of Ophthamology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • T. Shimada
    Department of Biochemistry and Molecular Biology,
    Nippon Medical School, Bunkyo–Ku, Japan
  • S. Oikawa
    Department of Third internal medicine,
    Nippon Medical School, Bunkyo–Ku, Japan
  • Footnotes
    Commercial Relationships  H. Oharazawa, None; T. Igarashi, None; T. Kurai, None; H. Matsui, None; T. Shiwa, None; H. Takahashi, None; K. Ohara, None; T. Shimada, None; S. Oikawa, None.
  • Footnotes
    Support  TAKEDA SCIENCE FOUNDATION
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 1883. doi:
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      H. Oharazawa, T. Igarashi, T. Kurai, H. Matsui, T. Shiwa, H. Takahashi, K. Ohara, T. Shimada, S. Oikawa; Identification of gene expression profiling associated with angiogenesis in human retinal endothelial cells compared with different endothelial cells. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1883.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Diabetic retinopathy is one of the most serious vascular complications of long–term diabetes. This pathologic angiogenesis is characterized by extensive proliferation of new blood vessels in the retina. However, pathogenic angiogenesis have not formed in other tissues, such as skin microvascular endothelial cells. To inhibit retinal neovascularization is necessary for speculation of characterizations in retinal endothelial cells compared with different endothelial cells. In this study, we investigated to identify the gene expression profiling associated with angiogenesis in three kinds of endothelial cells. Methods: We used three kinds of cultured microvascular endotherial cells, human retinal endothelial cells (HRECs), human skin microvascular endothelial cells (HMVECs) and human umbilical vein endothelial cells (HUVECs). Total RNA were isolated from these cells in normal cultured condition. Isolated total RNA was used for cDNAarray containing 96 known cDNA fragments associated with angiogenesis. The images detected from cDNAarray were analysed by subtractive densitometry. Results: Vascular endothelial growth factor D (VEGF–D), neuropilin 1, fibroblast growth factor 6 (FGF–6), vascular endothelial cadherin (VE–cadherin), CD31, Endoglin, fibronectin–1, integrin alpha5, secreted protein, acidic, and rich in cysteine (SPARC), macrophage scavenger receptor 1 (MSR–1), transforming growth factor–beta receptor 2 (TGFBR–2), TGFBR3 and thrombospondin 1 (TSP–1) in HREC tended to be higher than those in HUVEC and HMVEC. Conclusions: We have identified the differences of gene expression profiling associated with angiogenesis among three types of endothelial cells. Particular interest was highly gene expression of VEGF–D and neuropilin 1 in HRECs, but another VEGF and VEGF receptors was not expressed. VEGF receptors might be disappeared for passage the cultured cells. This preliminary study can play important roles to clarify the retinal angiogenesis. This research was supported by TAKEDA SCIENCE FOUNDATION.

Keywords: diabetic retinopathy • gene/expression • retinal neovascularization 
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