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A. Iriyama, S. Uchida, Y. Yanagi, Y. Tamaki, Y. Inoue, K. Matsuura, M. Araie, K. Kadonosono; Effects of Indocyanine Green on Retinal Ganglion Cells . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1978.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Recently, indocyanine green (ICG) has been used to visualize the internal limiting membrane during vitrectomy. After the intraocular administration of ICG, it accumulates to the optic disk. In this study, the effects of ICG on retinal ganglion cells (RGCs) were examined. Methods:In vitro, rat RGCs were purified by a two–step immunopanning procedure and briefly exposed to ICG (2.5 x 103 mg/L) and irradiated with an endoilluminator for 15 minutes or incubated in the presence of ICG (concentration ranging from 2 to 250 mg/L) without irradiation. The number of viable RGCs was counted after 3 days in culture. In vivo, after rats received an intravitreal injection of 3 µL ICG (0.25 and 2.5 mg/L), the distribution of ICG was observed with a fundus camera, and the number of viable RGCs was examined by DiI– retrolabeling technique. Results:In vitro, a brief exposure to ICG and light did not affect the RGC survival. However, ICG reduced the number of viable RGCs in a dose–dependent manner when exposed for three days. In vivo, the dye initially distributed on the retinal surface and around the optic disk. At day 7, the fluorescence became invisible in the 0.25 mg/L group, whereas the staining of the optic disk contour was evident in the 2.5 mg/L group. The number of the viable RGCs was significantly decreased in the 2.5 mg/L group 14 days after the injection. Conclusions:ICG showed an inherent toxicity to RGCs. The lowest concentration and the shortest staining time of ICG should be used for dye–assisted vitrectomy.
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