May 2004
Volume 45, Issue 13
ARVO Annual Meeting Abstract  |   May 2004
Large vitreous biopsy using perfluorocarbon perfused vitrectomy (PCPV)
Author Affiliations & Notes
  • S. Solis–Bermudez
    Retina, APEC, Mexico, Mexico
  • J.O. Rivera–Sempertegui
    Retina, APEC, Mexico, Mexico
  • G. Ortega–Larrocea
    Retina, APEC, Mexico, Mexico
  • P. Navarro–Lopez
    Retina, APEC, Mexico, Mexico
  • C. Leizaola–Fernandez
    Retina, APEC, Mexico, Mexico
  • H. Quiroz–Mercado
    Retina, APEC, Mexico, Mexico
  • Footnotes
    Commercial Relationships  S. Solis–Bermudez, None; J.O. Rivera–Sempertegui, None; G. Ortega–Larrocea, None; P. Navarro–Lopez, None; C. Leizaola–Fernandez, None; H. Quiroz–Mercado, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2025. doi:
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      S. Solis–Bermudez, J.O. Rivera–Sempertegui, G. Ortega–Larrocea, P. Navarro–Lopez, C. Leizaola–Fernandez, H. Quiroz–Mercado; Large vitreous biopsy using perfluorocarbon perfused vitrectomy (PCPV) . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2025.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:To evaluate PCPV as an alternative method to obtain large vitreous biopsy. Methods:: Balanced saline solution infusion was replaced by an infusion of perfluorocarbon liquid (PCL). As PCL was injected through the infusion port during pars–plana vitrectomy, vitreous debris and PCL were aspirated from the aspiration line of vitrectomy probe using a 10cc syringe. Since PCL are immiscible in bodily fluids a clear interface was observed between vitreous and PCL. Once vitrectomy was finished, separation of vitreous from the syringe was easy by maintaining it in a vertical position and pushing the piston of the syringe. Results:Three eyes with chronic uveitis without definitive diagnosis were included. More than 3 cc of vitreous were obtained in each case. Five milliliters of PCL were enough to obtain large vitreous biopsy. Mixture of vitreous and PCL was not observed. A clear vitreous cavity was maintained during vitrectomy and vitreous cavity was isolated by the presence of PCL. Final diagnosis was Candida albicans in two cases and Herpes simplex on the other. Conclusions:PCPV is a useful method to obtain large vitreous biopsy. Additional advantages of the method included a clear visualization and isolation of vitreous cavity. PCPV may have potential as a therapeutic procedure

Keywords: vitreoretinal surgery • retina • clinical (human) or epidemiologic studies: systems/equipment/techniques 

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