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S. Solis–Bermudez, J.O. Rivera–Sempertegui, G. Ortega–Larrocea, P. Navarro–Lopez, C. Leizaola–Fernandez, H. Quiroz–Mercado; Large vitreous biopsy using perfluorocarbon perfused vitrectomy (PCPV) . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2025.
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Purpose:To evaluate PCPV as an alternative method to obtain large vitreous biopsy. Methods:: Balanced saline solution infusion was replaced by an infusion of perfluorocarbon liquid (PCL). As PCL was injected through the infusion port during pars–plana vitrectomy, vitreous debris and PCL were aspirated from the aspiration line of vitrectomy probe using a 10cc syringe. Since PCL are immiscible in bodily fluids a clear interface was observed between vitreous and PCL. Once vitrectomy was finished, separation of vitreous from the syringe was easy by maintaining it in a vertical position and pushing the piston of the syringe. Results:Three eyes with chronic uveitis without definitive diagnosis were included. More than 3 cc of vitreous were obtained in each case. Five milliliters of PCL were enough to obtain large vitreous biopsy. Mixture of vitreous and PCL was not observed. A clear vitreous cavity was maintained during vitrectomy and vitreous cavity was isolated by the presence of PCL. Final diagnosis was Candida albicans in two cases and Herpes simplex on the other. Conclusions:PCPV is a useful method to obtain large vitreous biopsy. Additional advantages of the method included a clear visualization and isolation of vitreous cavity. PCPV may have potential as a therapeutic procedure
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