May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Pigment epithelial–derived factor (PEDF) inhibits apoptosis in a rat model of retinal detachment
Author Affiliations & Notes
  • L. Sobrin
    Retina Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • T.A. Young
    Retina Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • A. Sharma
    Retina Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • T. Nakazawa
    Retina Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • C.L. Grosskreutz
    Glaucoma Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • D.N. Zacks
    Retina Service, Kellogg Eye Center, Ann Arbor, MI
  • J.W. Miller
    Retina Service,
    Massachusetts Eye and Ear Infirmary, Boston, MA
  • Footnotes
    Commercial Relationships  L. Sobrin, None; T.A. Young, None; A. Sharma, None; T. Nakazawa, None; C.L. Grosskreutz, None; D.N. Zacks, Massachusetts Eye and Ear Infirmary P; J.W. Miller, Massachusetts Eye and Ear Infirmary P.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2064. doi:
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      L. Sobrin, T.A. Young, A. Sharma, T. Nakazawa, C.L. Grosskreutz, D.N. Zacks, J.W. Miller; Pigment epithelial–derived factor (PEDF) inhibits apoptosis in a rat model of retinal detachment . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2064.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To determine if pigment epithelial–derived factor (PEDF) prevents apoptosis in a rat model of retinal detachment. Methods: Retinal detachments were created in Brown Norway rats by injecting 10% hyaluronic acid into the subretinal space using a transvitreous approach. PEDF (2.5 µg or 5.0 µg) was injected into the vitreous (n = 9 eyes) or subretinal space (n = 8 eyes) immediately following the creation of the detachment. Vehicle (n = 3 eyes) or nothing (n = 3 eyes) was injected into the vitreous or subretinal space in control animals. All eyes were enucleated 72 hours after retinal detachment induction. The enucleated globes were embedded in paraffin and sectioned every 4 µm. Light microscopy and terminal dUTP–biotin nick end–labeling (TUNEL) was performed to assess for morphologic features associated with apoptosis. TUNEL–positive cells were counted per mm of tissue by a masked observer. The mean number of TUNEL–positive cells per mm of tissue was determined by averaging the number of TUNEL–positive cells per mm of tissue in three sections of each eye. Results: Light microscopic analysis of detached retinas showed the presence of pyknotic nuclei in the outer nuclear layer and disruption of the normal organization of the photoreceptor outer segments. TUNEL–staining was positive in the outer nuclear layer only in the detached portions of retina. Preliminary results show that the number of TUNEL–positive cells per mm of tissue was greater in subretinal injection control eyes (mean ± SD, 63.8 ± 11.9) vs. eyes injected subretinally with PEDF 2.5 µg (17.6 ± 15.5) or PEDF 5.0 µg (30.4 ± 18.1). These differences were statistically significant by two–tailed t–test (p = 0.007 and p = 0.016, respectively). There was no statistically significant difference in the number of TUNEL–positive cells found in the intravitreal–injected controls vs. PEDF–injected eyes. Conclusions: These preliminary data suggest that PEDF injected subretinally reduces apoptosis in a rat model of retinal detachment. PEDF and other neuroprotective agents may have a role in protecting photoreceptors in disorders characterized by a detachment of the neurosensory retina.

Keywords: retinal detachment • apoptosis/cell death • photoreceptors 
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