May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Increased Expression of VEGF in Retinal Pigmented Epithelial (RPE) Cells is Not Sufficient to Cause Choroidal Neovascularization (CNV)
Y. Oshima; S. Oshima; H. Nambu; S. Kachi; S.F. Hackett; M. Kaleko; S. Connelly; N. Esumi; D.J. Zack; P.A. Campochiaro
Author Affiliations & Notes
  • Y. Oshima
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
    Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
  • S. Oshima
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • H. Nambu
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • S. Kachi
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • S.F. Hackett
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • M. Kaleko
    Advanced Vision Therapies, Inc., Rockville, MD
  • S. Connelly
    Advanced Vision Therapies, Inc., Rockville, MD
  • N. Esumi
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • D.J. Zack
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • P.A. Campochiaro
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD
  • Footnotes
    Commercial Relationships  Y. Oshima, None; S. Oshima, None; H. Nambu, None; S. Kachi, None; S.F. Hackett, None; M. Kaleko, Advanced Vision Therapies, Inc. E; S. Connelly, Advanced Vision Therapies, Inc. E; N. Esumi, None; D.J. Zack, None; P.A. Campochiaro, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2229. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Increased Expression of VEGF in Retinal Pigmented Epithelial (RPE) Cells is Not Sufficient to Cause Choroidal Neovascularization (CNV)
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Y. Oshima, S. Oshima, H. Nambu, S. Kachi, S.F. Hackett, M. Kaleko, S. Connelly, N. Esumi, D.J. Zack, P.A. Campochiaro; Increased Expression of VEGF in Retinal Pigmented Epithelial (RPE) Cells is Not Sufficient to Cause Choroidal Neovascularization (CNV) . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2229.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: Increased expression of vascular endothelial growth factor (VEGF) in the retina is sufficient to stimulate sprouting of neovascularization (NV) from the deep capillary bed of the retina, but not the superficial retinal capillaries or the choriocapillaris. Coexpression of VEGF and angiopoietin 2 (Ang2) results in sprouting of NV from superficial and deep retinal capillaries, but not the choriocapillaris. In this study, we used the human VMD2 (vitelliform macular dystrophy 2) promoter, an RPE–specific promoter, combined with the tetracycline–inducible promoter system, to generate double transgenic mice with inducible expression of VEGF in RPE cells. Methods: Double transgenic mice with doxycycline–inducible expression of VEGF in RPE cells (VMD2/rtTA–TRE/VEGF mice) and triple transgenics (VMD2/rtTA–TRE/VEGF–TRE/Ang2) with inducible expression of both VEGF and Ang2 in RPE cells were generated. Subretinal injections of gutless adenoviral vectors expressing Ang2 (AGVAng2) were performed as previously described. Results: Adult mice with increased expression of VEGF in RPE cells had normal retinas and choroids with no CNV, but when increased expression of VEGF in RPE cells was combined with subretinal injection of AGVAng2, CNV consistently occurred. In contrast, triple transgenic mice with induced expression of Ang2 and VEGF in RPE cells, did not develop CNV. Conclusions: These data suggest that increased expression of VEGF and/or Ang2 in RPE cells is not sufficient to cause CNV unless it is combined with a subretinal injection of a gutless adenoviral vector, which is likely to perturb RPE cells. These data also suggest that the effects of angiogenic proteins may vary among vascular beds, even those that are closely related, and therefore generalizations should be avoided.

Keywords: choroid: neovascularization • retinal neovascularization • transgenics/knock–outs 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept