May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Spectral analysis of retinal vessels without blood
Author Affiliations & Notes
  • R.A. Chipman
    Optical Sciences Center,
    University of Arizona, Tucson, AZ
  • N. Beaudry
    Optical Sciences Center,
    University of Arizona, Tucson, AZ
  • S. Liston
    Medicine,
    University of Arizona, Tucson, AZ
  • R. Park
    Opthamology,
    University of Arizona, Tucson, AZ
  • D. Salyer
    Optical Sciences Center,
    University of Arizona, Tucson, AZ
  • K. Twietmeyer
    Optical Sciences Center,
    University of Arizona, Tucson, AZ
  • Footnotes
    Commercial Relationships  R.A. Chipman, None; N. Beaudry, None; S. Liston, None; R. Park, None; D. Salyer, None; K. Twietmeyer, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2403. doi:
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    • Get Citation

      R.A. Chipman, N. Beaudry, S. Liston, R. Park, D. Salyer, K. Twietmeyer; Spectral analysis of retinal vessels without blood . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2403.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To characterize the transmission spectra of saline filled retinal arteries and veins and the light scattering properties of retinal vessels. Methods: The central retinal artery of an enucleated swine eye is cannulated and the retinal circulation flushed with heparinized saline solution. The eyes are illuminated with a scanning monochromator coupled into a modified fundus camera. Hyperspectral images of nearly clear retinal vessels are acquired across the visible spectrum. The eyes are then dissected and retinal vessel pairs mounted on microscope slides and additional hyperspectral images are acquired. Results: Hyperspectral fundus images of saline filled retinal vessels are shown below (the blood filled vein is a result of backwash). Transmission spectra of empty vessels show no clear absorption features in the visible. Because blood filled vessels are brighter than the fundus background in the red, we postulate an enhanced scattering mechanism is operating in this spectral region. Conclusions: Retinal vessel spectra are useful for retinal light propagation modeling particularly for simulating retinal contrast in the red.  

Keywords: retina 
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