May 2004
Volume 45, Issue 13
ARVO Annual Meeting Abstract  |   May 2004
Expression of VEGF and PEDF in a Rat Model of ROP
Author Affiliations & Notes
  • J.R. McColm
    Ophthalmology, University of North Carolina, Chapel Hill, NC
  • P. Geisen
    Ophthalmology, University of North Carolina, Chapel Hill, NC
  • M.E. Hartnett
    Ophthalmology, University of North Carolina, Chapel Hill, NC
  • Footnotes
    Commercial Relationships  J.R. McColm, None; P. Geisen, None; M.E. Hartnett, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2494. doi:
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      J.R. McColm, P. Geisen, M.E. Hartnett; Expression of VEGF and PEDF in a Rat Model of ROP . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2494.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To determine the expression of VEGF and PEDF in a rat model of ROP. Methods: A well defined rat model of ROP was used that cycled oxygen between 50% and 10% every 24 hours for 14 days. Newborn litters were subjected to this oxygen regime for various time periods and then euthanized. Controls were room air raised. Retinas were dissected from the animals and flat mounted and stained with Griffonia lectin to visualize the vasculature, stored in RNAlater for RT–PCR or in M–Per for ELISA. Results: This model results in an avascular peripheral retina at p14 (mean area 30%) and if the animals are left in room air for 4 more days, typical neovascularization is seen at the vascular avascular interface. Results are given in the table (units for the RT–PCR are arbitrary units based on densitometry standardized to 18S RNA). 

NT=not tested Conclusions:Animals raised in variable oxygen express VEGF and PEDF differently from room air raised animals, with periods of hyperoxia resulting in down–regulation of VEGF and up–regulation of PEDF, and periods of hypoxia resulting in the opposite effect. This may explain, in part, the differences seen in retinal vascularization seen in variable oxygen raised animals.

Keywords: growth factors/growth factor receptors • retina • retinopathy of prematurity 

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