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Y. Hirata, H. Nishiwaki, S. Miura, Y. Ieki, Y. Honda; Analysis of choriocapillaris flow patterns by continuous laser–targeted angiography in monkeys . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2596.
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Purpose:To investigate choriocapillaris flow patterns and its segmental distribution in monkeys by continuous laser–targeted angiography (LTA). Methods: A slit lamp was modified to incorporate two kinds of lasers (argon and diode). Carboxyfluorescein was encapsulated in heat–sensitive liposomes and injected intravenously. Encapsulated carboxyfluorescein was released locally by applying continuous heat beam provided by diode laser (810nm). Video angiograms were generated with excitation illumination provided by argon laser (488+514nm) to observe selective images of choriocapillaris. Results: By continuous application of diode laser, three distinct phases (filling, plateau and draining) of fluorescent images of choriocapillaris were observed. In the plateau phase, a cluster of lobules fed by a common arteriole was uniformly illuminated and this defined area did not change in size while continuous diode laser was applied to the same spot. Only in posterior regions, the filling and draining phases demonstrated that each lobule was filled from a central spot and drained along a peripheral ring, demonstrating honeycomb flow patterns. In peripheral regions, not only choriocapillaris but also large choroidal vessels were observed. Conclusions:Continuous LTA demonstrated clusters of lobules fed by a common arteriole and each cluster was found to be functionally independent. We demonstrated regional differences of choriocapillaris flow patterns and it was thought to support that the choriocapillaris in posterior regions provides a more highly efficient system of outflow than that in peripheral regions. Our modified LTA method appears to be useful in analyzing choroidal circulation in vivo.
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