May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
HMG–CoA REDUCTASE INHIBITOR( NK–104 ) ATTENUATES LEUKOCYTE–ENDOTHELIAL INTERACTION INDUCED BY ISCHEMIA–REPERFUSION INJURY IN THE RAT RETINA
Author Affiliations & Notes
  • K. Miyaki
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • K. Tomida
    Ophthalmology, Kamo Hospital, Toyota, Japan
  • A. Nishiwaki
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • A. Matubara
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • H. Morita
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • H. Hirata
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • M. Yoshida
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • Y. Ogura
    Ophthalmology, Nagoya City University, Nagoya, Japan
  • Footnotes
    Commercial Relationships  K. Miyaki, None; K. Tomida, None; A. Nishiwaki, None; A. Matubara, None; H. Morita, None; H. Hirata, None; M. Yoshida, None; Y. Ogura, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2605. doi:https://doi.org/
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      K. Miyaki, K. Tomida, A. Nishiwaki, A. Matubara, H. Morita, H. Hirata, M. Yoshida, Y. Ogura; HMG–CoA REDUCTASE INHIBITOR( NK–104 ) ATTENUATES LEUKOCYTE–ENDOTHELIAL INTERACTION INDUCED BY ISCHEMIA–REPERFUSION INJURY IN THE RAT RETINA . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2605. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Statins, 3–hydroxy–methylglutaryl coenzyme A reductase inhibitors, have been shown to lower serum cholesterol levels in clinical use. It is also reported that statins exert pleiotropic and beneficial effects on vascular endothelium. We investigated the effects of NK–104, one of the newly synthesized statins, on leukocyte accumulation during ischemia–reperfusion injury . Methods: Transient retinal ischemia was induced in Long Evans rats for 60 minutes using temporal ligation of the optic nerve. NK–104 was administered 5 minutes before the induction of retinal ischemia. Leukocyte–endothelial interactions in the post–ischemic retina were evaluated in vivo with a scanning laser ophthalmoscope. We evaluated the number of rolling leukocytes, the number of accumulated leukocytes and the diameters of major retinal artery and vein. P–selectin and intercellular adhesion molecule–1(ICAM–1) mRNA expression in the retina were semiquantitatively studied with RT–PCR method. Results:The NK–104 treated rats showed less arterial narrowing (66.8% P<0.05)compared with vehicle–treated rats. Venous dilation was inhibited in the NK–104 –treated rats (85.5% P<0.05)as compared to vehicle–treated rats. The number of the rolling leukocytes in the retinal veins of the NK–104 treated rats was reduced to 69.1% of vehicle–treated rats. The number of accumulated leukocytes in the NK–104 –treated rats was reduced to 68.7% of those in vehicle–treated rats. (P<0.05) The NK–104 treatment decreased ICAM–1 expression, while the treatment did not reduced P–selectin expression in the retina. Conclusions:NK–104 effectively attenuated ischemia–induced leukocyte–endothelial interactions in the rat retina after ischemia.

Keywords: retina 
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