May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Fibronectin is Highly Induced by Dexamethasone in Cultured Human Lens Epithelial Cells
Author Affiliations & Notes
  • C.Y. Im
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • C.Y. Kim
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • J.H. Lee
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • K.Y. Seo
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • H.K. Lee
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • E.S. Lee
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • S.H. Shin
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
  • E.–K. Kim
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ College of Med, Seoul, Republic of Korea
    Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  C.Y. Im, None; C.Y. Kim, None; J.H. Lee, None; K.Y. Seo, None; H.K. Lee, None; E.S. Lee, None; S.H. Shin, None; E. Kim, None.
  • Footnotes
    Support  02–PJ1–PG1–CH02–0003
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2651. doi:
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      C.Y. Im, C.Y. Kim, J.H. Lee, K.Y. Seo, H.K. Lee, E.S. Lee, S.H. Shin, E.–K. Kim; Fibronectin is Highly Induced by Dexamethasone in Cultured Human Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2651.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Fibronectin is known to have significant cell migration activity and is associated with epithelium mesenchymal transition. Therefore, we investigated level of fibronectin in human lens epithelial cell line (HLE–B3) treated with dexamethasone . Methods: Human lens epithelial cells were cultured in MEM with charcoal stripped 20% FBS. Dexamethasone was treated with different concentration of 0, 10–9M, 10–7M, 10–5M. After 72 hours, the level of fibronectin was examined with reverse transcription– polymerase chain reaction and western blot analysis. Results: The level of fibronectin was increased in a dose dependent manner by RT–PCR. Western blot also showed dose dependent increase of fibronectin There was no remarkable morphological change between no treated and different concentration of dexamethasone cells. Conclusions: The level of fibronectin is increased in cultured HLE–B3 cells treated with dexamethasone in a dose dependent manner. These data might indicate that the aberrant increase of fibronectin might play a role in steroid induced cataract. Further study is required to investigate the possible role of fibronectin in steroid induced cataract.

Keywords: cataract • extracellular matrix • aging 
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