May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Expression of mRNA associated with Fas–mediated apoptosis signaling is downregulated in Behcet’s patients with active uveitis
Author Affiliations & Notes
  • M. Takeuchi
    Ophthalmology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • A. Takeuchi
    Ophthalmology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • Y. Usui
    Ophthalmology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • M. Kuroda
    Pathology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • K. Yoshida
    Pathology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • M. Usui
    Ophthalmology,
    Tokyo Medical University, Shinjuku–ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships  M. Takeuchi, None; A. Takeuchi, None; Y. Usui, None; M. Kuroda, None; K. Yoshida, None; M. Usui, None.
  • Footnotes
    Support  Grant in Aid for Scientific Research 14770978
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2700. doi:
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      M. Takeuchi, A. Takeuchi, Y. Usui, M. Kuroda, K. Yoshida, M. Usui; Expression of mRNA associated with Fas–mediated apoptosis signaling is downregulated in Behcet’s patients with active uveitis . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2700.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Behcet's disease (BD) is a multisystemic inflammatory disorder, characterized by diverse and complex immune abnormalities and causes relapsing ocular inflammation. In this study, we attempted to characterize BD by the specific gene expression proliles of peripheral blood mononuclear cells (PBMC) using DNA microarray. Methods: The gene expression profiles of 20,000 potentially relevant genes were analyzed by the Codelink Bioarray using peripheral blood mononuclear cells (PBMC) from 13 Behcet’s patients with active ocular inflammation and 11 controls. Results: Compared to mean mRNA expression of controls, 569 genes were identified which exhibited at least 2–fold increases or decreases expression in all BD patients (163 up–regulated and 406 down–regulated). Among them, although mRNA expression of NFkB, tumor necrosis factor (ligand) superfamily member 13B (TNFSF13B), TNFSF12A, nerve growth factor receptor (NGFR), and paired box gene 6 (PAX6) were exclusively up–regulated, mRNA expression of caspase 2, caspase 3, caspase 8, APO–1/CD95 (FAS)–associated phosphatase, regulator of Fas–induced apoptosis, and FAS (TNFRSF6)–associated via death domain (FADD) were constitutively down–regulated. Conclusions: While expression of mRNA associated with inflammatory process and with eye development were promoted, mRNA expression involved in Fas–mediated apoptosis signaling was constitutively inhibited in BD patients with active uveitis.

Keywords: uveitis–clinical/animal model • gene microarray • inflammation 
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