May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The Effect of Doxycycline on the Migration of Pterygial Epithelial Cells grown in Culture
Author Affiliations & Notes
  • C.A. Cox
    Section of Molecular Therapeutics,
    National Eye Institute, Bethesda, MD
  • M. John–Aryankalayil
    Section of Molecular Therapeutics,
    National Eye Institute, Bethesda, MD
  • C. Jaworski
    Section of Molecular Therapeutics,
    National Eye Institute, Bethesda, MD
  • P. Russell
    Aging and Ocular Diseases,
    National Eye Institute, Bethesda, MD
  • T. Gray
    Ophthalmology and Visual Sciences and Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX
  • K. Freedman
    Ophthalmology and Visual Sciences and Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX
  • N. Dushku
    Ophthalmology, Kaiser Permanente Medical Center, Sacramento, CA
  • T.W. Reid
    Ophthalmology and Visual Sciences and Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX
  • D. Carper
    Section of Molecular Therapeutics,
    National Eye Institute, Bethesda, MD
  • Footnotes
    Commercial Relationships  C.A. Cox, None; M. John–Aryankalayil, None; C. Jaworski, None; P. Russell, None; T. Gray, None; K. Freedman, None; N. Dushku, None; T.W. Reid, None; D. Carper, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2939. doi:
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      C.A. Cox, M. John–Aryankalayil, C. Jaworski, P. Russell, T. Gray, K. Freedman, N. Dushku, T.W. Reid, D. Carper; The Effect of Doxycycline on the Migration of Pterygial Epithelial Cells grown in Culture . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2939.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To determine if doxycycline, a matrix metalloproteinase inhibitor (MMPI), inhibits the migration of cultured pterygial epithelial cells. Methods: The migration of third–passage pterygial epithelial cells grown in culture was measured with and without doxycycline of different concentrations using the quantitative fibronectin cell migration Boyden Chamber assay. The control cells were grown under the same conditions as the treated cells (serum–free Dulbecco's medium containing 5% bovine serum albumin) except without doxycycline. The extent of migration was measured colorimetrically, and the data were analyzed using the student's t–test and analysis of variance (ANOVA). Results: Doxycycline (500 µg/ml) significantly decreased migration when compared to control (P < 0.0002). Doxycycline at the lower concentrations (50 µg/ml and 5 µg/ml) showed a trend toward decreasing migration. There was no significant difference between control sets. Conclusions: Doxycycline significantly decreased pterygial cell migration when compared to the control when used at the 500 µg/ml concentration. This may eventually be beneficial to humans in reducing the angiogenesis and growth of pterygia, and may therefore prove to be vision–saving.

Keywords: Pterygium • pharmacology • extracellular matrix 
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