May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The effect of apolipoprotein J/ clusterin on retinal endothelial cell death after retinal ischemia in the rat
J. Kim; J. Kim; S. Jeong; Y. Choi; K.–W. Kim; Y.S. Yu
Author Affiliations & Notes
  • J. Kim
    Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
    Wonmi Public Health Center, Bucheon city, Gyeonggi–do, Republic of Korea
  • J. Kim
    Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • S. Jeong
    Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Y. Choi
    Pharmacy, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Republic of Korea
  • K.–W. Kim
    Pharmacy, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Republic of Korea
  • Y.S. Yu
    Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Kim, None; J. Kim, None; S. Jeong, None; Y. Choi, None; K. Kim, None; Y.S. Yu, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3265. doi:
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      J. Kim, J. Kim, S. Jeong, Y. Choi, K.–W. Kim, Y.S. Yu; The effect of apolipoprotein J/ clusterin on retinal endothelial cell death after retinal ischemia in the rat . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3265.

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Abstract

Abstract: : Purpose: To determine the effect of clusterin on retinal endothelial cell in ischemic retina Methods: At 6, 12, 24, 48 hours after permanent ipsilateral carotid artery ligation of the Sprague–Dawley rats, immunohistochemistry and western blotting for clusterin, PECAM, and ZO–1, and TUNEL were performed in the retina. MTT assay was done in hydrogen peroxide – treated cells with clusterin overexpression. Results: In the ipsilateral retina, clusterin expression was progressively enhanced with increasing time. Clusterin was prominently expressed in inner retina. In contrast, PECAM and ZO–1 were inversely decreased. TUNEL was evident at 24 hours. Clusterin overexpression did not upregulate cell death. Conclusions: Ischemia affects the expression of clusterin in the retina. Clusterin expression may reflect an ischemia–induced damage and/or may protect retinal endothelial cell from ischemic injury.

Keywords: ischemia • vascular cells • cell death/apoptosis 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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