May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Intercellular Adhesion Molecule–1 Expression And Effects Of Superoxide Dismutase During Retinal Ischemia Reperfusion Injury In Hypertensive Rats.
Author Affiliations & Notes
  • F. Hirose
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • J. Kiryu
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • K. Miyamoto
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • K. Nishijima
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • S. Miyahara
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • H. Katsuta
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • H. Tamura
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • K. Musashi
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • Y. Honda
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • Footnotes
    Commercial Relationships  F. Hirose, None; J. Kiryu, None; K. Miyamoto, None; K. Nishijima, None; S. Miyahara, None; H. Katsuta, None; H. Tamura, None; K. Musashi, None; Y. Honda, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3282. doi:
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      F. Hirose, J. Kiryu, K. Miyamoto, K. Nishijima, S. Miyahara, H. Katsuta, H. Tamura, K. Musashi, Y. Honda; Intercellular Adhesion Molecule–1 Expression And Effects Of Superoxide Dismutase During Retinal Ischemia Reperfusion Injury In Hypertensive Rats. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3282.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:A number of studies have suggested that hypertension affects the pathogenesis of inflammatory reactions and oxidative stress of ischemia reperfusion injury in various organs. The objective of this study was to evaluate the effects of hypertension on leukocyte–endothelial interactions and Intercellular adhesion molecule–1 (ICAM–1) expression during retinal ischemia reperfusion injury. Methods:Transient retinal ischemia for 60 minutes was induced in spontaneously hypertensive rats (SHR) and age–matched normotensive Wistar–Kyoto rats (WKY) by ligation of the optic nerve. At 4, 12, 24, 48 and 72hours after reperfusion, flat mounts of retina were prepared to evaluate the density of leukocytes that had been accumulated in the retinal microcirculation using fluorescence microscopy. ICAM–1 mRNA expression in the postischemic retina was studied by semiquantitative polymerase chain reaction. At 14 days after reperfusion, ischemia–induced retinal damage and the effect of superoxide dismutase (SOD) on the damage was evaluated histologically. Results:In the SHR group, the number of accumulated leukocytes peaked at 48 hours after reperfusion, and it was increased to 5.2–fold, as compared with that of the WKY group (P<0.001). ICAM–1 mRNA expression was upregulated significantly in postischemic retina in SHR compared with WKY (P<0.01). Histologic examination demonstrated marked increase in the ischemia–induced retinal damage in SHR (P<0.01) and a significant amelioration of the damage by treatment with SOD in SHR (P<0.05). Conclusions:In the SHR group, the leukocyte–endothelial cell interactions and the histologic retinal damage after transient ischemia increased compared with those seen in the WKY group. Oxidative stress may thus be an important mechanism for the deterioration seen in retinal ischemia reperfusion injury in SHR.

Keywords: cell adhesions/cell junctions • hypoxia • oxidation/oxidative or free radical damage 
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