May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Dectection of Argyrophilic Nucleolar Organizer Regions (AgNORs) and p53 and Ki–67 Immunoreactivity in Ocular Squamous Epithelial Tumors in the Horse
C.B. Mosunic; P.A. Moore; E. Howerth; U.M. Dietrich; M. Chandler; K.P. Carmichel
Author Affiliations & Notes
  • C.B. Mosunic
    Dept of Small Animal Medicine,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • P.A. Moore
    Dept of Small Animal Medicine,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • E. Howerth
    Dept of Pathology,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • U.M. Dietrich
    Dept of Small Animal Medicine,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • M. Chandler
    Dept of Small Animal Medicine,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • K.P. Carmichel
    Dept of Pathology,
    University of Georgia, College of Veterinary Medicine, Athens, GA
  • Footnotes
    Commercial Relationships  C.B. Mosunic, None; P.A. Moore, None; E. Howerth, None; U.M. Dietrich, None; M. Chandler, None; K.P. Carmichel, None.
  • Footnotes
    Support  Companion Animal Fund, University of Georgia
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3565. doi:
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      C.B. Mosunic, P.A. Moore, E. Howerth, U.M. Dietrich, M. Chandler, K.P. Carmichel; Dectection of Argyrophilic Nucleolar Organizer Regions (AgNORs) and p53 and Ki–67 Immunoreactivity in Ocular Squamous Epithelial Tumors in the Horse . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3565.

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Abstract

Abstract: : Purpose: Equine ocular squamous epithelial neoplasms represent a continuum from the benign squamous plaque to malignant squamous cell carcinoma in situ and finally the invasive squamous cell carcinoma. Each of these forms of neoplasia represents a different prognosis and makes accurate diagnosis essential. The purpose of this study was to utililize AgNOR scoring as well as p53 and Ki–67 immunohistochemistry as diagnostic tools to determine histopathologically the difference between borderline cases of squamous epithelial tumors. Methods: Archived samples of equine ocular squamous epithelial tumors were retrieved. AgNOR scoring, and p53 and Ki–67 immunohistochemistry was performed. AgNOR scores were obtained from 48 samples (22 invasive squamous cell carcinomas, 10 squamous cell carcinoma in situ, and 16 squamous plaques). p53 immunohistochemistry was performed on 46 samples (22 invasive squamous cell carcinomas, 9 squamous cell carcinoma in situ, and 15 squamous plaque). Ki–67 immunohistochemisty was performed on 38 samples (19 invasive squamous cell carcinomas, 8 squamous cell carcinoma in situ, and 11 squamous plaque). The mean AgNOR and mean immunoreactivity scores were compared for statistical significance. Results: The mean AgNOR score was significantly different between squamous cell carcinoma and squamous plaque (p<0.0001) and squamous cell carcinoma in situ (p<0.0001). Mean AgNOR score was also significantly different between squamous cell carcinoma in situ and squamous plaque (p=0.0005). The mean p53 immunoreactivity score was significantly different between squamous cell carcinoma and squamous plaque (p<0.0001) and squamous cell carcinoma in situ and squamous plaque (p=0.0006). The mean Ki–67 immunoreactivity score was significantly different between squamous cell carcinoma and squamous plaque (p<0.0004). Conclusion: AgNOR score is a useful diagnostic tool in discriminating between borderline cases of squamous epithelial tumors. p53 immunoreactivity also proved to be helpful, especially in discriminating between squamous plaque and squamous cell carcinoma in situ. Ki–67 in our study was not a helpful histological diagnostic tool.

Keywords: tumors • pathology techniques • immunohistochemistry 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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