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M. Tei, K. Kusaba, S. Kinoshita; Experimental model of retinal vein stasis using a vitreous injection of bFGF–impregnated gelatin microsperes in the rabbit. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3595.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To establish a new model of retinal vein stasis in the rabbit by the injection of basic fibroblast growth factor (bFGF)–impregnated gelatin microspheres into a vitreous cavity. Methods: 10–20 µm size of bFGF–impregnated gelatin microsperes, prepared by forming a polyion complex between gelatin and bFGF, were injected into rabbit vitreous cavity. Three pigmented rabbits weighing approximately 2 kg were anesthetized with intramuscular Ketamine and Xylazine injection. The microsperes, containing 50 micrograms of bFGF, were injected in the vitreous close to the optic disc. Ophthalmoscopic examinations were performed every 2 days for 2 weeks and every 1 week for 8 weeks. Fluorescein angiography was performed at 7 and 14 days after the bFGF–impregnated gelatin microsperes injection. Control eyes received bFGF–free gelatin microspheres. Results: Obvious vein dilation and tortuosity and mild artery dilation were observed for 14 days in all bFGF–impregnated gelatin microsperes injected eyes. Neovascularization was not observed in any of the bFGF–impregnated gelatin microsperes injected eyes. Fluorescein angiography showed delayed retinal circulation. Control eyes showed neither vein dilation nor vein tortuosity. Conclusions: Intravitreal injection of bFGF–impregnated gelatin microspheres induces temporal venous out flow disturbance from the eye in rabbits. This model resembles retinal vein occlusion. It could be useful for evaluating therapies for central retinal vein occlusion.
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