May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Experimental model of retinal vein stasis using a vitreous injection of bFGF–impregnated gelatin microsperes in the rabbit.
Author Affiliations & Notes
  • M. Tei
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo–Ku, Japan
  • K. Kusaba
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo–Ku, Japan
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo–Ku, Japan
  • Footnotes
    Commercial Relationships  M. Tei, None; K. Kusaba, None; S. Kinoshita, None.
  • Footnotes
    Support  Japanese MHW and ME (30116024)
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3595. doi:
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      M. Tei, K. Kusaba, S. Kinoshita; Experimental model of retinal vein stasis using a vitreous injection of bFGF–impregnated gelatin microsperes in the rabbit. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3595.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To establish a new model of retinal vein stasis in the rabbit by the injection of basic fibroblast growth factor (bFGF)–impregnated gelatin microspheres into a vitreous cavity. Methods: 10–20 µm size of bFGF–impregnated gelatin microsperes, prepared by forming a polyion complex between gelatin and bFGF, were injected into rabbit vitreous cavity. Three pigmented rabbits weighing approximately 2 kg were anesthetized with intramuscular Ketamine and Xylazine injection. The microsperes, containing 50 micrograms of bFGF, were injected in the vitreous close to the optic disc. Ophthalmoscopic examinations were performed every 2 days for 2 weeks and every 1 week for 8 weeks. Fluorescein angiography was performed at 7 and 14 days after the bFGF–impregnated gelatin microsperes injection. Control eyes received bFGF–free gelatin microspheres. Results: Obvious vein dilation and tortuosity and mild artery dilation were observed for 14 days in all bFGF–impregnated gelatin microsperes injected eyes. Neovascularization was not observed in any of the bFGF–impregnated gelatin microsperes injected eyes. Fluorescein angiography showed delayed retinal circulation. Control eyes showed neither vein dilation nor vein tortuosity. Conclusions: Intravitreal injection of bFGF–impregnated gelatin microspheres induces temporal venous out flow disturbance from the eye in rabbits. This model resembles retinal vein occlusion. It could be useful for evaluating therapies for central retinal vein occlusion.

Keywords: retina • vascular occlusion/vascular occlusive disease • drug toxicity/drug effects 
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