Coexpression of fibrillin–1 and versican in chick iris and ciliary body

Abstract

Abstract: : Purpose: To determine the presence of both fibrillin–1 and versican in chick iris and ciliary body by using immunohistochemical and biochemical methods. Methods: We performed RT–PCR on RNAs extracted from chick iris and ciliary body. Immunofluorescence stainings and Immunoblots were performed on chick iris and ciliary body samples using antibodies against versican and fibrillin–1. Results: We identified the colocalization of fibrillin–1 and versican at the levels of mRNA and protein in chick iris and ciliary body. Conclusions: Here we showed the coexpression of fibrillin–1 and versican in chick iris and ciliary body. These molecules are presumably bound covalently as shown previously, and this binding system may be important for the iris and ciliary body as the reservoir of versican.

Keywords: extracellular matrix • proteoglycans/glycosaminoglycans • ciliary body