May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
TGF–beta 1 concentration is increased after UVB irradiation in hTERT immortalized human fibroblasts
Author Affiliations & Notes
  • S. Jung
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ. College of Med, Seoul, Republic of Korea
    Brain Korea 21 Project for Medical Science, Yonsei Univ., Seoul, Republic of Korea
  • S. Choi
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ. College of Med, Seoul, Republic of Korea
  • E.–K. Kim
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ. College of Med, Seoul, Republic of Korea
    Brain Korea 21 Project for Medical Science, Yonsei Univ., Seoul, Republic of Korea
  • C.Y. Im
    Institute of Vision Research, Dept. Ophthalmology, Yonsei Univ. College of Med, Seoul, Republic of Korea
  • J. Park
    Daegu Yonsei Eye Clinic, Daegu, Republic of Korea
  • J.V. Jester
    Dept. Ophthalmology, Univ. Texas Southwestern Medical Center, Dallas, TX
  • Footnotes
    Commercial Relationships  S. Jung, None; S. Choi, None; E. Kim, None; C.Y. Im, None; J. Park, None; J.V. Jester, None.
  • Footnotes
    Support  02–PJ1–PG1–CH02–0003
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3783. doi:
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    • Get Citation

      S. Jung, S. Choi, E.–K. Kim, C.Y. Im, J. Park, J.V. Jester; TGF–beta 1 concentration is increased after UVB irradiation in hTERT immortalized human fibroblasts . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3783.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The Avellino corneal dystrophy (ACD), one of the Big–H3 gene related dystrophy, begins at the interpapebral fissure where cornea is exposed to sun–light even after squint. As Big–H3 gene is activated by TGF–beta, the concentration of TGF–beta 1 was checked from the culture media after UVB irradiation of the keratocytes to find out the possible relation between ACD and UVB. Methods: Immotalized human fibroblasts (hTERT, extended–life human corneal fibroblasts) were cultured with DMEM containing 10% FBS and irradiated with 0, 5, 10, 15 and 30 mJ/cm2 UVB. Culture supernatants were collected at 0, 4, 8, 24, and 48 hours after UVB irradiation and TGF–beta 1 production was assayed by Enzyme–Linked Immunosorbant Assay (ELISA). Analysis of co–variance (ANCOVA) was performed after log transformation of TGF–beta 1 values. Results: Production of TGF–beta 1 protein in culture supernatants assayed by ELISA was increased at 10, 15 and 30 mJ/cm2 UVB irradiation (p<0.05). Conclusions: UVB irradiation increases the level of TGF–beta 1 in the corneal stroma. Increased TFG–beta 1 would activate Big–H3 gene to produce more abnormal keratoepithelin and may be a factor in the progression of ACD. TGF–beta 1 concentration (pg/ml) after UVB irradiation 

Keywords: cornea: stroma and keratocytes • trauma • aging 
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