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Y. Okada, S. Saika, T. Miyamoto, K. Shirai, Y. Ohnishi, T. Ueyama, E. Senba; AP–1 expression in ethanol–treated corneal epithelium in vivo . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3809.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the expression pattern of stress–related genes, c–fos and c–jun, both the major components of AP–1, in rat corneal epithelium treated by a short–term ethanol exposure. The purpose of the currect study was to examine if the ethanol exposure during LASEK may stimulate/damage the corneal epithelial cells. Method: Thirty male Wistar rats were used. Fifty µl 20% ethanol was placed onto the central corneal epithelium in 2.4mm diameter for 30 seconds. The affected eyes were then enucleated after various intervals of healing after being washed with saline. Frozen sections were processed for in situ hybridization with c–fos and c–jun mRNAs or were stained with anti–c–Fos and anti–c–Jun antibodies. Results: Thirty to 60 min after the treatment, signals for c–fos and c–jun mRNAs were detected in the corneal epithelium. These signals were no longer evident at 90 min. c–Fos protein was detected in the corneal epithelium around the area of ethanol exposure from 60 to 120 min after the treatment. But, no immunoreactivity for c–Jun was detected in the epithelium. Conclusion: Corneal epithelial cells are transiently transcriptionally activated at a very early phase after the ethanol exposure. The epithelium surrounding the ethanol–exposed area is markedly activated. mRNA expression for c–fos is followed by protein synthesis, but that of c–jun is not.
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