May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Tear Proteins in Patients with Rosacea
Author Affiliations & Notes
  • L.S. Alvarenga
    Ophthalmology,
    University of California, Davis, CA
    Ophthalmology, Federal University of Sao Paulo, UNIFESP, Sao Paulo, Brazil
  • E.N. Martins
    Ophthalmology,
    University of California, Davis, CA
    Ophthalmology, Federal University of Sao Paulo, UNIFESP, Sao Paulo, Brazil
  • Z. Smit–McBride
    Biological Chemistry,
    University of California, Davis, CA
  • D.K. Hsu
    Dermatology,
    University of California, Davis, CA
  • C.D. Reilly
    Ophthalmology,
    University of California, Davis, CA
  • L.M. Hjelmeland
    Ophthalmology,
    Biological Chemistry,
    University of California, Davis, CA
  • M.J. Mannis
    Ophthalmology,
    University of California, Davis, CA
  • Footnotes
    Commercial Relationships  L.S. Alvarenga, None; E.N. Martins, None; Z. Smit–McBride, None; D.K. Hsu, None; C.D. Reilly, None; L.M. Hjelmeland, None; M.J. Mannis, None.
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3881. doi:
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      L.S. Alvarenga, E.N. Martins, Z. Smit–McBride, D.K. Hsu, C.D. Reilly, L.M. Hjelmeland, M.J. Mannis; Tear Proteins in Patients with Rosacea . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3881.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Qualitative and quantitative determination of tear fluid components is of increasing interest in ophthalmology, because it can lead to a better understanding of the pathogenesis of ocular surface diseases and aid in diagnosing and/or monitoring treatment. In this study we analyzed tear proteins of rosacea patients. Methods: Tears from rosacea patients (N=5) and controls (N=5) were obtained by conjunctival stimulation with paper (Schirmer) strips and were collected with glass capillary tubes. Samples were processed using lab–on–a–chip technology (Agilent 2100 Bioanalyzer / Protein 200 LabChip kit) in reducing conditions (2–betamercaptoethanol). Tear proteins were grouped according to molecular weight in seven groups (<20, 21–40, 41–60, 61–80, 81–100, 101–120, >120 KDa). Data demonstrated a skewed distribution, and groups were compared with a non–parametric test (Mann–Whitney). Results: The median concentration of tear proteins groups in rosacea patients were: <20 KDa = 1334.7 µg/ml (828.7–5947–6); 21–40 KDa = 1852.7 µg/ml (359.4–6870.5); 41–60 KDa= 501.9 µg/ml (179.5–7218.5); 61–80 KDa = 76.2 µg/ml (0–346.6); 81–100 KDa=247.5 µg/ml (237–1413.6); 101–120 KDa = 1523.2 µg/ml (209.2–3518–7); > 120 KDa = 114.5 µg/ml (0–150.2). The control group showed significantly lower concentration of proteins with 40–60 KDa ( P=0.0472). We did not detect a difference when the other molecular weight groups were compared. Conclusions: Rosacea patients present a distinct tear protein profile with a higher concentration of proteins with molecular weight between 41–60 Kda when compared tto normal controls.

Keywords: anterior segment • cornea: tears/tear film/dry eye 
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