May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Characteristics of Lyophilized Human Amniotic Membrane
Author Affiliations & Notes
  • T. Rodriguez–Ares
    Ophthalmology,
    University of Santiago, Santiago de Compostela, Spain
  • M.J. López–Valladares
    Ophthalmology,
    University of Santiago, Santiago de Compostela, Spain
  • R. Touriño
    Ophthalmology,
    University of Santiago, Santiago de Compostela, Spain
  • M. Silva
    Institute of Orthopedics and Tissue Bank,
    University of Santiago, Santiago de Compostela, Spain
  • E. Becerra
    Anatomopathology,
    University of Santiago, Santiago de Compostela, Spain
  • B. Vieites
    Anatomopathology,
    University of Santiago, Santiago de Compostela, Spain
  • Footnotes
    Commercial Relationships  T. Rodriguez–Ares, None; M.J. López–Valladares, None; R. Touriño, None; M. Silva, None; E. Becerra, None; B. Vieites, None.
  • Footnotes
    Support  Supported by FIS–PI021608
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3918. doi:
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    • Get Citation

      T. Rodriguez–Ares, M.J. López–Valladares, R. Touriño, M. Silva, E. Becerra, B. Vieites; Characteristics of Lyophilized Human Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3918.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To describe histological changes observed in human amniotic membrane preserved by freeze–drying, as part of a study to assess freeze–drying as a method to preserve amniotic membrane. Methods: Human placentas were obtained aster elective cesarean deliveries from donors seronegative. After being washed and separated from corion, amniotic membrane was laid onto a cellulose nitrate filter and cut in pieces. This pieces went under lyophilization following a program previously validated (freeze–dryer VIRTIS Genesis). Small samples of freeze–dried human amniotic membrane were examined with light microscope after hematoxylin and eosine dye. To estimate cellular viability amniotic membranes were immersed in propidium iodide and calcein AM. Results: Histopathological exam showed no significant morphologic alteration after freeze–drying in human amniotic membrane, keeping epithelial cells and basement membrane with their characteristics. The observation with the fluorescence microscope demonstrated that there was not cellular viability. Conclusions: Freeze–drying keeps histological structure of human amniotic membrane and could be a good alternative as a preservation method for human amniotic membrane until surgery, though more studies are necessary to determine what happen with the concentration of cytokines in which lay most of the observed clinical effects of human amniotic membrane.

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