May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Reconstruction of Lamellar Bioartificial Cornea Using Lyophilized Amniotic Membrane and Collagen scaffold
J.–I. Ahn; I.–K. Jang; J.–C. Kim; K.–Y. Song; E.–K. Yang; J.–K. Park
Author Affiliations & Notes
  • J.–I. Ahn
    Chemical & Biochemical Engineering, Dongguk University, Seoul, Republic of Korea
    Ophthalmology, Chung–Ang University Hospital, Seoul, Republic of Korea
  • I.–K. Jang
    Chemical & Biochemical Engineering, Dongguk University, Seoul, Republic of Korea
    Ophthalmology, Chung–Ang University Hospital, Seoul, Republic of Korea
  • J.–C. Kim
    Ophthalmology, Chung–Ang University Hospital, Seoul, Republic of Korea
  • K.–Y. Song
    Pathology, Chung–Ang University College of Medicine, Seoul, Republic of Korea
  • E.–K. Yang
    R&D Center, Bioland Ltd., Chung–nam, Republic of Korea
  • J.–K. Park
    Chemical & Biochemical Engineering, Dongguk University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Ahn, None; I. Jang, None; J. Kim, None; K. Song, None; E. Yang, None; J. Park, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3925. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Reconstruction of Lamellar Bioartificial Cornea Using Lyophilized Amniotic Membrane and Collagen scaffold
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      J.–I. Ahn, I.–K. Jang, J.–C. Kim, K.–Y. Song, E.–K. Yang, J.–K. Park; Reconstruction of Lamellar Bioartificial Cornea Using Lyophilized Amniotic Membrane and Collagen scaffold . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3925.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose:Evaluation of the possibility that lyophilized amniotic membrane could be used as a substratum for the reconstruction of bioartificial cornea. Methods:Amniotic membrane (AM) was prepared by cryopreservation (CAM) or lyophilization (LAM). Before the cell cultivation, AM was placed in the tissue holding device. We reconstructed corneal epithelium on LAM and CAM. We manufactured composite scaffold (AM sponge) to co–culture corneal epithelial cells and fibroblasts to make a lamellar bioartificial cornea (LBC). AM sponge was fabricated by attaching the collagen sponge to the stromal side of LAM. To reconstruct LBC, corneal fibroblasts were inoculated into a collagen scaffold, and cultivated for 6 days. And then corneal epithelial cells were inoculated on LAM. To evaluate the reconstructed corneal epithelium and LBC, we stained the reconstructed tissue sections against PCNA, P63, CK3, type IV collagen and TEM was also examined. Results:LAM and CAM had similar properties of attachment, stratification and differentiation of corneal epithelial cells under histological and immunohistological examination. Corneal epithelium and stromal layer could be successfully reconstructed using AM sponge. The morphology and protein expressions on the reconstructed corneal epithelium were similar to normal cornea. Basement membrane and basal layer of reconstructed epithelium had positive expression to type IV collagen, PCNA, and P63. The expression of CK3 in the superficial epithelium of reconstructed tissue was also similar to that of normal cornea. And we found the desmosomal contacts between corneal epithelial cells and the hemidesmosomal contacts between corneal epithelial cells and AM. Conclusions:Corneal epithelium and LBC were successfully reconstructed using LAM and collagen scaffold. The reconstructed corneal epithelium and lamellar bioartificial cornea could be used for treating the patients with severely damaged cornea.

Keywords: cornea: epithelium • cornea: stroma and keratocytes 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept