May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
A bioengineered human oral epithelial cell sheet using a temperature–responsive culture surface for autologous ocular surface reconstruction
Y. Hayashida; K. Nishida; M. Yamato; K. Watanabe; K. Yamamoto; N. Maeda; H. Watanabe; A. Kikuchi; T. Okano; Y. Tano
Author Affiliations & Notes
  • Y. Hayashida
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • K. Nishida
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • M. Yamato
    Biomedical Engineering, Tokyo Women's University, Shinjuku, Japan
  • K. Watanabe
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • K. Yamamoto
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • N. Maeda
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • H. Watanabe
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • A. Kikuchi
    Biomedical Engineering, Tokyo Women's University, Shinjuku, Japan
  • T. Okano
    Biomedical Engineering, Tokyo Women's University, Shinjuku, Japan
  • Y. Tano
    Ophthalmology, Osaka Univ Medical School, Suita, Japan
  • Footnotes
    Commercial Relationships  Y. Hayashida, None; K. Nishida, Cell Seed C, P; M. Yamato, Cell Seed C, P; K. Watanabe, None; K. Yamamoto, None; N. Maeda, None; H. Watanabe, None; A. Kikuchi, None; T. Okano, Cell Seed C, P; Y. Tano, None.
  • Footnotes
    Support  Grant (15390530) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3929. doi:
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      Y. Hayashida, K. Nishida, M. Yamato, K. Watanabe, K. Yamamoto, N. Maeda, H. Watanabe, A. Kikuchi, T. Okano, Y. Tano; A bioengineered human oral epithelial cell sheet using a temperature–responsive culture surface for autologous ocular surface reconstruction . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3929.

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Abstract

Abstract: : Purpose: Last year we reported our autologous transplantation of a bioengineered, cultivated oral epithelial cell sheet using a temperature–responsive culture surface for ocular surface reconstruction in rabbits (ARVO 2002). In this study, we describe the development of cultivated human oral mucosal epithelial cell sheets using temperature–responsive cell culture surfaces. Methods: Oral mucosal epithelial cells from a healthy volunteer were cultured on a temperature–responsive culture surface with 3T3 feeder cells for two weeks. Cultivated cell sheets were then harvested by reducing the temperature. The sheets were observed by histologic analyses, including light and electron microscopy and immunohistochemistry. Results: A cultivated sheet of human oral epithelial cells could be harvested intact from the culture surfaces as a clear tissue construct simply by reducing the temperature without the need for enzymatic treatment. The bioengineered cell sheet was composed of three to five cell layers with tight junctions and desmosomes. Cornea–specific keratin K3 was expressed as in human corneal epithelium. Conclusions: The human oral epithelial cell sheets fabricated in our culture system are morphologically similar to the human corneal epithelium in vivo. The results suggest promising clinical applications for our bioengineered oral epithelial sheet ideal for transplantation.

Keywords: cornea: epithelium • transplantation • regeneration 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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