May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Collagen–immobilized poly (vinyl alcohol) hydrogel as an artificial cornea scaffold.
H. Miyashita; S. Shimmura; H. Kobayashi; T. Taguchi; N. Kato; A. Usui; J. Shimazaki; J. Tanaka; K. Tsubota
Author Affiliations & Notes
  • H. Miyashita
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • S. Shimmura
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • H. Kobayashi
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Japan
  • T. Taguchi
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Japan
  • N. Kato
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • A. Usui
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • J. Shimazaki
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • J. Tanaka
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Japan
  • K. Tsubota
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • Footnotes
    Commercial Relationships  H. Miyashita, None; S. Shimmura, None; H. Kobayashi, None; T. Taguchi, None; N. Kato, None; A. Usui, None; J. Shimazaki, None; J. Tanaka, None; K. Tsubota, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3934. doi:
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      H. Miyashita, S. Shimmura, H. Kobayashi, T. Taguchi, N. Kato, A. Usui, J. Shimazaki, J. Tanaka, K. Tsubota; Collagen–immobilized poly (vinyl alcohol) hydrogel as an artificial cornea scaffold. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3934.

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Abstract

Abstract: : Purpose: To investigate the feasibility of using collagen–immobilized poly (vinyl alcohol) (PVA) hydrogel as an artificial cornea scaffold. Methods: Human limbal epithelial cells were air–lift cultured on collagen–immobilized PVA with 3T3 feeder layer cells for 2 weeks. Differentiation of the epithelium and basement membrane formation were examined by electron microscopy and immnohistochemistry. Barrier function of the epithelium was studied by horseradish peroxidase (HRP) permeability assay. The collagen–immobilized PVA with stratified rabbit epithelium was implanted into rabbits as lamellar grafts. Implants were observed by slit lamp for 4 weeks, and subsequently histological change was analyzed. Results: Human limbal cells on the collagen–immobilized PVA formed 3 to 5–layer thick keratin–3–positive epithelium. HRP/diaminobenzidine reaction products were mainly observed on the surface of the epithelium after HRP permeability assay. The PVA polymer was easy to handle, and suturable onto the corneal surface. Although the polymer caused little tissue reaction, epithelial defects were often observed. Conclusions: Collagen–immobolized PVA scaffolds support a stratified human corneal epithelium in vitro. Biocompatibility of PVA in lamellar implants showed that PVA has the potential to be used as an artificial cornea substrate.

Keywords: keratoprostheses • cornea: basic science • cornea: epithelium 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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