May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Influence of vancomycin on adhesion of a Staphylococcus epidermidis strain encoding ica locus to intraocular lenses
Author Affiliations & Notes
  • L. Kodjikian
    Ophthalmology, Croix–Rousse Hospital, Lyon, France
    "Biomaterials and Matrix Remodeling", EA 3090,
    Claude Bernard University, Lyon, France
  • F.N. R. Renaud
    "Biomaterials and Matrix Remodeling", EA 3090,
    Claude Bernard University, Lyon, France
    Microbiology, Institute of Pharmaceutical and Biological Sciences, Lyon, France
  • C. Roques
    Microbiology, EA 819, Xenobiotics Kinetics, Pharmacy Faculty, Paul Sabatier University,, Toulouse, France
  • J.G. Garweg
    Ophthalmology, Inselspital, Bern University, Bern, Switzerland
  • G. Pellon
    Biochemistry,
    Claude Bernard University, Lyon, France
  • J. Freney
    "Biomaterials and Matrix Remodeling", EA 3090,
    Claude Bernard University, Lyon, France
    Microbiology, Institute of Pharmaceutical and Biological Sciences, Lyon, France
  • C. Burillon
    "Biomaterials and Matrix Remodeling", EA 3090,
    Claude Bernard University, Lyon, France
    Ophthalmology, Ed Herriot Hospital, Lyon, France
  • Footnotes
    Commercial Relationships  L. Kodjikian, None; F.N.R. Renaud, None; C. Roques, None; J.G. Garweg, None; G. Pellon, None; J. Freney, None; C. Burillon, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 3997. doi:
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      L. Kodjikian, F.N. R. Renaud, C. Roques, J.G. Garweg, G. Pellon, J. Freney, C. Burillon; Influence of vancomycin on adhesion of a Staphylococcus epidermidis strain encoding ica locus to intraocular lenses . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3997.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To assess anti–adhesion and/or bactericidal properties of vancomycin in vitro and to determine exactly when these effects are detectable, in order to estimate its relevance to perioperative antibiotic prophylaxis. To also analyze the efficiency of a newly designed vancomycin insert prototype for endophthalmitis prevention. Methods: Staphylococcus epidermidis clinical strain N890074, containing the intercellular adhesion (ica) locus, was used as infectious agent. Vancomycin was used at 20 µg/ml. A sterile biocompatible biodegradable vancomycin insert, releasing 230 µ g of antibiotics over 100 minutes, was designed especially for this study. To obtain bacterial killing curves, the first experiments were performed in a 103 CFU/ml bacterial suspension containing no intraocular lenses (IOL). Then IOLs were incubated in the suspension and bacterial adherence was determined using bacterial counting, with and without antibiotic. Results:Vancomycin (solution and insert) had an anti–adhesion effect after 1 hour and a relevant bactericidal effect after 6 hours of incubation. Conclusions:Vancomycin used along with irrigating solutions does not remain in the anterior chamber long enough to develop any bactericidal effect. Even if it initially reduces bacterial adhesion, used with a drug level dropping below the bacterial minimal inhibitory concentration, it could result in a secondary increase of adhesion of slime–producing bacteria. A sufficiently high concentration was obtained by the new sustained release system thereby overcoming the drawback of a short half life within the anterior chamber and allowing a clinically satisfying anti–adhesion and bactericidal action of vancomycin.

Keywords: endophthalmitis • pharmacology • antibiotics/antifungals/antiparasitics 
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