May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Intrinsic choroidal neurons (ICN) in human: evidence for the presence of both nitrergic and non–nitrergic subpopulations
Author Affiliations & Notes
  • A. Bergua
    Department of Ophthalmology, Physiology/Histology,
    University Erlangen–Nuernberg, Erlangen, Germany
  • A. Juenemann
    Department of Ophthalmology, Physiology/Histology,
    University Erlangen–Nuernberg, Erlangen, Germany
  • A. De Laet
    Department of Ophthalmology, Physiology/Histology,
    RUCA University, Antwerp, Belgium
  • J.–P. Timmermans
    Anatomy I, Histology,
    RUCA University, Antwerp, Belgium
  • M.–J. Tassignon
    Ophthalmology, University Hospital Antwerp, Edegem, Belgium
  • A. Brehmer
    Anatomy I, Histology,
    University Erlangen–Nuernberg, Erlangen, Germany
  • W.L. Neuhuber
    Anatomy I, Histology,
    University Erlangen–Nuernberg, Erlangen, Germany
  • F. Schroedl
    Anatomy I, Histology,
    University Erlangen–Nuernberg, Erlangen, Germany
  • Footnotes
    Commercial Relationships  A. Bergua, None; A. Juenemann, None; A. De Laet, None; J. Timmermans, None; M. Tassignon, None; A. Brehmer, None; W.L. Neuhuber, None; F. Schroedl, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4287. doi:
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      A. Bergua, A. Juenemann, A. De Laet, J.–P. Timmermans, M.–J. Tassignon, A. Brehmer, W.L. Neuhuber, F. Schroedl; Intrinsic choroidal neurons (ICN) in human: evidence for the presence of both nitrergic and non–nitrergic subpopulations . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4287.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Nitrergic intrinsic choroidal neurons (ICN) innervate vascular and stromal non–vascular smooth muscle cells, hence influencing choroidal blood flow and choroidal thickness. Studies of ICN topography with different markers (NADPH–d: Bergua et al., 2003; neurofilament–staining: Trivino et al., 2002) revealed different ICN densities in temporal versus nasal quadrants, suggesting the presence of non–nitrergic ICN next to the nitrergic ones. Therefore, the aim of this study was to quantify these neurochemically distinct ICN subpopulations as well as to search for an overall marker of human ICN. Methods: Meeting the Declaration of Helsinki, 11 choroidal wholemounts from donor eyes (53–84 years of age; 1–16 hrs p.m.) were processed for single and double immuno/histochemical stainings for the following markers: NADPH–d, cocktail of neurofilament 160/200kD (NF), protein–gene product 9.5 (PGP9.5), and neuronal nitric oxide synthase (nNOS), respectively. For documentation, light–, fluorescence– and confocal laser scanning microscopy were used. Results: The combination of NADPH–d and PGP 9.5 revealed that about 6% of the total population of ICN were PGP 9.5+/NADPH–d– without preferential topography. Double labelling with NF and nNOS revealed, that about 20% of the total population of ICN were positive for NF+/nNOS– and about 6% were NF–/nNOS+, again without topographical preference. Conclusions: Our results show, that there exists a hitherto unrecognized NF+ non–nitrergic subpopulation of ICN, the chemical nature and targets of which have to be further elucidated.

Keywords: immunohistochemistry • neurotransmitters/neurotransmitter systems • neuropeptides 
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