May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Ex Vivo Study of the Effect of Intraocular Pressure on Transcorneal Penetration of Topically Applied Gentamicin Sulphate
Author Affiliations & Notes
  • M. Abdulrazik
    Pharmaceutics, Hebrew University of Jerusalem, adisacom@palnet.com, Jerusalem, Israel
  • Footnotes
    Commercial Relationships  M. Abdulrazik, Independent Inventor P.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4556. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Abdulrazik; Ex Vivo Study of the Effect of Intraocular Pressure on Transcorneal Penetration of Topically Applied Gentamicin Sulphate . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4556.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To study the effect of intraocular pressure changes on transcorneal penetration of topically applied drugs. Methods: Eight Albino rabbits (2.0–2.5 kg) were sacrificed and their eyes enucleated. The freshly excised corneas were mounted horizontally beneath a humidity controlled donor chamber and above a pressure controlled acceptor chamber of an ex vivo diffusion apparatus, with the endothelial side facing balanced salt solution (BSS–plus, Alcon Labs. Inc., Fort Worth, TX, USA) filled acceptor chamber. One microliter of 4% gentamicin as sulphate (Rafa Labs. Ltd., Jerusalem, Israel) was applied on the center of the epithelial surface of the mounted cornea at various pressure levels of acceptor chamber (0, 20, 40, 60 mm Hg; 4 corneas for each pressure level). After 60 minutes, the concentration of gentamicin in the acceptor chamber fluid was measured by fluorescence polarization immunoassay using a TDx System Analyzer (Abbott labs., Irving, TX, USA). Results: Best transcorneal gentamicin sulphate penetration was achieved at acceptor chamber pressure of 20 mm Hg, with less penetration at higher pressure levels. When the pressure in the acceptor chamber was lowered to the zero level, the drug penetration was relatively poor. Conclusions: Ex vivo transcorneal drug penetration studies should include transcorneal pressure setting that simulates physiological intraocular pressure.

Keywords: pharmacology • cornea: basic science • intraocular pressure 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×