May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Variants of CTACK (CCL27) are expressed in mouse eye
Author Affiliations & Notes
  • D. Ledee
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • I. Gery
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • P. Zelenka
    National Eye Institute, National Institutes of Health, Bethesda, MD
  • Footnotes
    Commercial Relationships  D. Ledee, None; I. Gery, None; P. Zelenka, None.
  • Footnotes
    Support  NEI Intramural Program
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4716. doi:
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      D. Ledee, I. Gery, P. Zelenka; Variants of CTACK (CCL27) are expressed in mouse eye . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4716.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: An RT–PCR based screen of mouse eye RNA detected mRNAs related to the chemokine, CTACK (CCL27), thought to be skin–specific. This study seeks to characterize these RNAs. Methods: Primers were synthesized corresponding to the 5' and 3' end of the reported CTACK mRNA sequence and RT–PCR was used to examine CTACK–related sequences in mouse eye RNA. 5'–RACE was used to examine sequences 5' of exon 2. Results: RT–PCR amplified three variants of CTACK: fully spliced (exons 1, 2, and 3), partially spliced (retaining intron 1) and unspliced (retaining intron 1 and 2). 5'–RACE predominantly amplified a previously reported CTACK variant, called PESKY, which differs from CTACK at the 5' end. Comparing the reported PESKY mRNA sequence with the mouse genomic DNA sequence revealed that the 5'–end of PESKY joins 2 upstream exons that are separated by a 2kb intron in the genomic sequence. 5'–RACE also detected a PESKY variant containing this intron. Conclusions: The eye contains multiple splice products related to CTACK, some of which may be eye–specific. The predominant RNA corresponds to PESKY, a non–secreted chemokine that is targeted to the nucleus and enhances cell motility. We also detect a novel, alternatively spliced variant of PESKY.

Keywords: cytokines/chemokines • gene/expression 
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