May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Gene transfer of anti–apoptotic and cytoprotective molecules in human corneal endothelial cells and their influence on prevention of apoptosis
Author Affiliations & Notes
  • T. Ritter
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • I. Ecke
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • J. Yang
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • M. Hempel
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • S. Metzner
    Department of Ophthalmology,
    Charite–University Medicine Berlin, Berlin, Germany
  • S. Schu
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • H.–D. Volk
    Inst of Medical Immunology,
    Charite–University Medicine Berlin, Berlin, Germany
  • U. Pleyer
    Department of Ophthalmology,
    Charite–University Medicine Berlin, Berlin, Germany
  • Footnotes
    Commercial Relationships  T. Ritter, None; I. Ecke, None; J. Yang, None; M. Hempel, None; S. Metzner, None; S. Schu, None; H. Volk, None; U. Pleyer, None.
  • Footnotes
    Support  Supported by DFG PL 150/10–1
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4794. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Ritter, I. Ecke, J. Yang, M. Hempel, S. Metzner, S. Schu, H.–D. Volk, U. Pleyer; Gene transfer of anti–apoptotic and cytoprotective molecules in human corneal endothelial cells and their influence on prevention of apoptosis . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4794.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Corneal transplantation (keratoplasty) is the most often performed allo–transplantation. The loss of endothelial cells during ex–vivo storage of the cornea before grafting is a major complication. Apoptotic cell death seems to play a role during this process. The aim of our work is to investigate whether gene transfer of anti–apoptotic and cytoprotective genes prevents the induction of apoptosis in human corneal endothelial cells (HCEC). Methods: We used a replication–deficient (E1/E3–deleted) adenovirus (Ad) vector to deliver the anti–apoptotic genes bcl–xL, bag–1 and the cytoprotective gene heme oxygenase–1 (HO–1) to HCEC. The influence of gene transfer on the survival rate of endothelial cells after induction of apoptosis with different stimuli (TNF–alpha (100ng/ml)/Cycloheximid (20µg/ml), Camptothecin (5µM), Staurosporine (0,5µM)) has been examined by two different techniques (detection of Caspase 3, 7 and TUNEL–assay). Results: Adbcl–xL gene transfer is able to protect HCEC from induction of apoptosis in all experiments investigated (p=0.01 vs. non–transduced HCEC). Interestingly AdHO–1 and Adbag–1 gene transfer only protects the cells from Camptothecin induced apoptosis but not from TNF–alpha/Cycloheximid and Staurosporine induced apoptosis. Conclusions: Our results indicate that Bcl–xL is a very effective inhibitor of apoptosis in HCEC and therefore is a promising candidate to improve the survival rate of corneal cells during storage and subsequent transplantation.

Keywords: apoptosis/cell death • cornea: endothelium • transplantation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×