May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Inhibition of VEGF Expression by VEGF RECEPTOR–1 Intrakine
Author Affiliations & Notes
  • S. Amin
    Ophthalmology, Medical College Georgia, Augusta, GA
  • N. Singh
    Ophthalmology, Medical College Georgia, Augusta, GA
  • E. Macnamara
    Ophthalmology, Medical College Georgia, Augusta, GA
  • A. Patel
    Ophthalmology, Medical College Georgia, Augusta, GA
  • J. Ambati
    Ophthalmology, Medical College Georgia, Augusta, GA
  • Z. Dong
    Ophthalmology, Medical College Georgia, Augusta, GA
  • J. Wang
    Ophthalmology, Medical College Georgia, Augusta, GA
  • B.K. Ambati
    Ophthalmology, Medical College Georgia, Augusta, GA
  • Footnotes
    Commercial Relationships  S. Amin, None; N. Singh, None; E. Macnamara, None; A. Patel, None; J. Ambati, None; Z. Dong, None; J. Wang, None; B.K. Ambati, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4818. doi:
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    • Get Citation

      S. Amin, N. Singh, E. Macnamara, A. Patel, J. Ambati, Z. Dong, J. Wang, B.K. Ambati; Inhibition of VEGF Expression by VEGF RECEPTOR–1 Intrakine . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4818.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To determine if a VEGF receptor coupled with an endoplasmic retention signal can block hypoxia–induced upregulation of VEGF secretion in corneal epithelial cells. Methods:Human corneal epithelial cells were transfected with plasmids encoding the VEGF–retention signal protein. Successfully transfected cells were selected for using G418. Two days later, transfected cells were subjected to 5% hypoxia for 24h. Supernatant was sampled at 24h and assayed for VEGF by ELISA. Results:Baseline VEGF concentration was 286 pg/mL; this increased to 457 pg/mL at 24h, an increase of 60%. The endoplasmic reticulum retention signal coupled VEGF receptor was able to limit the increase of VEGF secretion to 18% at 24h (p<0.05). Conclusions:Endoplasmic reticulum retention signal coupled VEGF receptors can significantly suppress hypoxia–induced VEGF secretion by corneal epithelial cells.

Keywords: cornea: epithelium • vascular occlusion/vascular occlusive disease • cornea: basic science 
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