May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Effect of Mast Cell Hypertrophy and Deficiency on Ocular Allergy
Author Affiliations & Notes
  • D. Miyazaki
    Higashisumiyoshi–ku, Japan
  • Footnotes
    Commercial Relationships  D. Miyazaki, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4846. doi:
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      D. Miyazaki; Effect of Mast Cell Hypertrophy and Deficiency on Ocular Allergy . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4846.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: When allergic symptoms occur, chronic stage of ocular allergy is generally intertwined with early phase and late phase reaction, where T cells, eosinophils, or other effectors could have significant influences. Mast cells are known as early phase mediator, however, relative contribution in the complicated inflammatory circumstances are not known. In this study, we sought to clarify how mast cells affect early phase allergic symptoms and examined whether mast cell deficiency may affect clinical outcomes. Methods:First, single allergen exposure model using NC/Nga, which induces mast cell hypertrophy, was used to analyze correlative factors with clinical symptoms. NC/Nga mice, a model of atopic dermatitis, was sensitized and challenged with short ragweed instillation 2 weeks post sensitization. Induced early phase symptoms 20 min after challenge (clinical score, sum of conjunctival edema/conjunctival redness/tearing/ lid swelling score, 0–16), inflammatory cells profile, and allergen specific serum IgE levels were analyzed. Second, repeated immunization scheme with multiple allergen exposure was used to evaluate effect of mast cell deficiency in early phase reaction. Mast cell deficient mice (KitW/KitW–v), sensitized with cat hair allergen (with weekly re–exposure) and challenged topically for 3 consecutive days 7 weeks post sensitization, were examined for early phase parameters. Results: In atopic model, clinical score significantly correlated with degranulated mast cell count (Spearman correlation analysis, ρ=0.52, p<0.05), but not with count of eosinophils or other subsets, showing early phase symptoms are mast cell degranulation dependent even in atopic situation. Moreover, the degranulated mast cell count showed linear correlation with allergen specific IgE levels (ρ=0.66, p<0.01), but not total IgE levels, supporting importance of specific IgE/FcεRI–mediated degranulation. To further analyze responsibility of mast cells for early phase symptoms, mast cell deficient mice (KitW/KitW–v) and mast cell competent wild type were analyzed under influence of late phase responses. In KitW/KitW–v, Allergen dependent early phase symptoms were absent. This phenomenon was also confirmed using Evans Blue dye extravasation. Conclusion: Mast cells are critical for early phase symptoms in ocular allergy.

Keywords: conjunctivitis • conjunctiva • cytokines/chemokines 
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