May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
cDNA Microarray Analysis of Gene Expression in Cultured Human Corneal Fibroblasts following TGF–ß2 Stimulation
K. Izumi; D. Kurosaka; Y. Mashima; T. Taya; N. Osada; Y. Oguchi; Y. Tanaka; T. Iwata
Author Affiliations & Notes
  • K. Izumi
    National Tokyo Medical Center, National Institute of Sensory Organs, Tokyo, Japan
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • D. Kurosaka
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Y. Mashima
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • T. Taya
    Life Sciences and Chemical Analysis Division, Agilent Technologies/Yokogawa Analytical Systems, Inc., Tokyo, Japan
  • N. Osada
    Life Sciences and Chemical Analysis Division, Agilent Technologies/Yokogawa Analytical Systems, Inc., Tokyo, Japan
  • Y. Oguchi
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Y. Tanaka
    National Tokyo Medical Center, National Institute of Sensory Organs, Tokyo, Japan
  • T. Iwata
    National Tokyo Medical Center, National Institute of Sensory Organs, Tokyo, Japan
  • Footnotes
    Commercial Relationships  K. Izumi, None; D. Kurosaka, None; Y. Mashima, None; T. Taya, Agilent Technologies/Yokogawa Analytical Systems, Inc. E; N. Osada, Agilent Technologies/Yokogawa Analytical Systems, Inc. E; Y. Oguchi, None; Y. Tanaka, None; T. Iwata, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 4875. doi:
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      K. Izumi, D. Kurosaka, Y. Mashima, T. Taya, N. Osada, Y. Oguchi, Y. Tanaka, T. Iwata; cDNA Microarray Analysis of Gene Expression in Cultured Human Corneal Fibroblasts following TGF–ß2 Stimulation . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4875.

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Abstract

Abstract: : Purpose:To identify changes in gene expression of human corneal fibroblasts after exposure to TGF–ß2. Methods:RNA was isolated from cultured human corneal fibroblasts after exposure to TGF–ß2 and hybridized to a human cDNA microarray containing 12,646 genes. Gene–specific semi–quantitative reverse transcription–polymerase chain reaction (RT–PCR) was used to confirm the gene expression pattern measured by the cDNA expression microarray (Agilent Technologies). Results:After exposure to TGF–ß2, the expression of 1,264 genes was significantly up–regulated and the expression of 762 genes was down–regulated in human corneal fibroblasts. Semi–quantitative RT–PCR analysis confirmed the TGF–ß2–mediated changes in expression patterns of these genes. Conclusions:We have analyzed the effects of gene expressions by TGF–ß2 treatment on human corneal fibroblasts using cDNA microarray. Transcription of genes coding cytoskeleton, ECM, and adhesion were significantly affected by TGF–ß2.

Keywords: gene microarray • cornea: stroma and keratocytes • wound healing 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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