Abstract
Abstract: :
Purpose:KV1.3 is a voltage–gated potassium channel, it has some effect in epithelium to modulate fluid secretion and take part in other physiological activity, but the mechanism of this affection is unclear. The purpose of this study was to investigate the effect of calcium involved in the course of voltage–gated potassium channel action. Methods:1. Bovine eye ciliary body was sectioned, and Kv1.3 antibody was labeled with standard immunoflurescence. 2. Primary cultures of NPE cell were isolated from bovine eye, rMargotoxin 5nM (blocker Kv1.3 channel), PBS, nifidipine and dantrolene, independent or combined to treat the cultured cells, and subcellular Ca2+ signaling patterns in these cells were examined by confocal line scanning microscopy. Results:The Kv1.3 signal were labeled in the apical PE and NPE cells. After blocked Kv1.3 channel, Ca2+ increased inside the cells, and this increase can be inhibited by nifidipine and dantrolene. Conclusions:kv1.3 can change the membrane potential to trigger calcium channel to modulate the action of ciliary epithelium.
Keywords: ciliary body • calcium • signal transduction: pharmacology/physiology