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C.K. Spee, C. Chinn, E. Barron, D.R. Hinton, S.J. Ryan; The Influence of Mouse Strain on the Development of Retinal Fibrosis. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5094.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To quantify the degree of retinal fibrosis in different strains of mice using a lens extraction model which induces proliferative vitreoretinopathy (PVR), (Invest Ophthalmol, 2003, program 3011). Methods: Adult C57Bl/6 and 129S3/SvImJ were anesthetized and an incision was made in the cornea with a razor blade and extended using iridectomy scissors. The crystalline lens was extracted out by gently applying pressure around the limbus. The vitreous cavity was filled with 1% hyaluronate, and Iso–Dent, an adhesive was used to close the corneal incision. In C57Bl/6 (n=10) and adult 129S3/SvImJ (n=10) mice, the eyes were enucleated at day 7 and fixed in 4% formalin. Whole mount sections of the entire eye were stained with collagen I antibody and the fibrotic membrane area was measured using confocal microscopy. Results: The mean area of the fibrotic membranes identified by Collagen I was 0.58 ± 0.38 mm2 in C57Bl/6(n=10) and 0.12 ± 0.028mm2 in 129SvImJ(n=10) mice, (p=0.0003). Conclusions: C57Bl/6 mice develop a more prominent fibrotic epiretinal membrane associated with significantly increased in collagen production as shown by the area difference between the two strains.
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