Purchase this article with an account.
K. Minamino, Y. Adachi, H. Yamada, A. Higuchi, Y. Suzuki, M. Iwasaki, Y. Zhang, M. Matsumura, S. Ikehara; A new strategy for transplantation of bone marrow stem cells into the retina. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5170.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: It has been reported that bone marrow cells (BMCs) develop into various kinds of cells. Recently, we have demonstrated that bone marrow stem cells (BMSCs) develop into retinal nerve cells, using low–density BMCs from 5FU–treated rats( Stem Cells. 2002;20:279–283). To apply this regeneration method clinically, the efficiency of transplantation of BMSCs into the retina is crucial. In this study, we show an efficient method to transplant BMSCs into the retina. Method: We injected BMSCs from EGFP–transgenic mice (C57BL/6 back ground) into the vitreous space or the subretinal space of untreated normal C57BL/6 mice and into the vitreous space of C57BL/6 mice, of which eyes had been pretreated with ischemia–reperfusion injury or light photocoagulation. Two weeks after transplantation, the mice were sacrificed. We observed frozen sections from eyeballs of the mice, using confocal microscopy, and counted EGFP–positive transplanted cells in the neurosensory retinae. Result: We detected very few transplanted cells in the retina, into which we had injected BMSCs without pretreatment. There was no statistical difference in the number of BMCs between the injection into the vitreous space or subretinal space. However, when we pretreated the eyes with ischemia–reperfusion injury or photocoagulation, we observed a significant number of transplanted cells in the neurosensory retina. There were statistical differences in the number of transplanted BMCs between normal vs photocoagulated, and normal vs ischemia–reperfusion. Conclusions: These results suggest that the retinal injury could induce various cytokines from damaged tissue and promote migration and differentiation of BMSCs, resulting in high–efficiency survival of transplanted cells.
This PDF is available to Subscribers Only